NOVELTY - Method for purifying triterpenoids of Ganoderma lucidum having high resistance with respect to human tumor cell activity, involves (a) extracting the G.lucidum, and concentrating extract, (b) eluting concentrate using macroporous resin column, (c) decompressing and spin-drying eluate, subjecting the eluate to primary purification, adding ethanol, and stirring mixture, (d) eluting the mixture using MCI GEL (RTM: Ion exchange column), (e) subjecting eluate to decolorization, and (f) concentrating and spray-drying product.    USE - The method is useful for purifying triterpenoids of G.lucidum which exhibits high resistance with respect to human tumor cell activity (claimed). The triterpenoid is used in drug for treating tumor.    ADVANTAGE - The method rapidly and cost-effectively purifies triterpenoids having high stability using non-toxic solvent, does not cause damage to human body and environment, and is suitable for industrial production.    DETAILED DESCRIPTION - Method for purifying triterpenoids of Ganoderma lucidum having high resistance with respect to human tumor cell activity, involves (a) extracting the G.lucidum using 0-20 vol.% ethanol, and concentrating extract, (b) passing concentrate through macroporous resin column at a flow rate of 0.5-5 BV/hour, eluting the macroporous resin containing concentrate using 4-8 times water at a flow rate of 2-6 BV/hour, then eluting the macroporous resin containing concentrate using 4-8 times ethanol (having concentration of 60-90 vol.%) at a flow rate of 1-4 BV/hour, collecting eluate, and removing ethanol from the eluate, (c) decompressing and spin-drying the eluate, subjecting the eluate to primary purification, adding 2-5 times ethanol to purified product, and stirring obtained mixture, (d) passing the mixture through MCI GEL (RTM: Ion exchange column), eluting the column using deionized water, then eluting the column using 6-8 times ethanol (having concentration of 10-30 vol.%), discarding eluate, eluting the column using 4-8 times ethanol (having concentration of 80-90 vol.%), and collecting eluate, (e) passing the eluate through polyamide column for decolorization to obtain decolorized liquid, and (f) concentrating the decolorized liquid, and spray-drying concentrate. The macroporous resin is D-101 macroporous resin or HPD-100 macroporous resin.