NOVELTY - Determining humus content involves using humus substances such as humic acid (HA), fulvic acid (FA) and humin (HM). The sampling is more at high temperature aerobic fermentation. The spectral characteristics of humic acid and fulvic acid are measured in each set of samples. The fermentation quality is analyzed by extracting humic acid and fulvic acid, and weighing air-dried sample. The solid-liquid is 1:20. The water bath extraction is carried out by 0.1mol/l sodium pyrophosphate tetrabasic and 0.1mol/l sodium hydroxide at 40 degrees C for 24 hours.    USE - Method for determining humus content (claimed).    ADVANTAGE - The method enables to determine humus content accurately by performing fermentation, and provides data to support the resource utilization.    DETAILED DESCRIPTION - Determining humus content involves using humus substances such as humic acid (HA), fulvic acid (FA) and humin (HM). The sampling is more at high temperature aerobic fermentation. The spectral characteristics of humic acid and fulvic acid are measured in each set of samples. The fermentation quality is analyzed by extracting humic acid and fulvic acid, and weighing air-dried sample. The solid-liquid is 1:20. The water bath extraction is carried out by 0.1mol/l sodium pyrophosphate tetrabasic and 0.1mol/l sodium hydroxide at 40 degrees C for 24 hours. The solution is centrifuged at 4000 revolutions per minute for 20 minutes. The supernatant is collected, and filtered by 0.45 mu m filter membrane. The obtained humic acid extract is washed with 6mol/l hydrochloric acid (pH 1-2), then placed at 60 degrees C for 4 hours. The solution comprising humic acid and fulvic acid is separated by centrifuging at 4000 revolutions per minute for 20 minutes to obtain humic acid precipitate. The precipitate is filtered to obtain extract and then washed with 0.1mol/l hydrochloric acid and 0.3mol/l hydrogen fluoride to obtain purified extract. The purified humic acid extract is dried at 80 degrees C and then sealed. The gel permeation chromatography analysis is performed by loading 303ml Dextran in glass column having filling height 67cm and inner diameter 25mm. 1ml HA 1ml FA extracts are added to the gel surface, then ultra-pure water (pH 6.8) is poured as the eluent using a peristaltic pump to control the flow rate that is 1ml/minute. The samples (5ml) are collected automatically, and measured with 1ml of 2% dextran solution. The absorbance of collected samples is measured at 280nm. The elution curve is drawn according to elution volume and corresponding absorbance. The fermentation maturity curve is determined on the basis of elution.