NOVELTY - Mixed culture composition comprises nutritional inorganic fertilizer, sawdust, oat hulls, litter, peanut shell, cottonseed hulls, calcium carbonate, corncob, bagasse, bran, corn flour, calcium sulfate, magnesium sulfate, lime, gypsum, urea comprising 46% nitrogen, triacontanol and soybean meal. The mixed culture composition has moisture content of 65 plus minus 1% and pH of 7.5-9. The nutritional inorganic fertilizer comprises boron fertilizer and potassium fertilizer.    USE - The mixed culture composition is useful for cultivating Pleurotus eryngii (claimed).    ADVANTAGE - The mixed culture composition is rich in vitamin B1, vitamin B, fiber, protein, mineral substance, microelements and other nutritional substances, cultivates edible P.eryngii, and promotes growth of P.eryngii which has good taste and provides good mouthfeel.    DETAILED DESCRIPTION - Mixed culture composition comprises 3-5 parts mass nutritional inorganic fertilizer, 60-70 parts mass sawdust, 20-90 parts mass oat hulls, 10-40 parts mass litter, 10-40 parts mass peanut shell, 5-15 parts mass cottonseed hulls, 26-30 parts mass calcium carbonate, 35-40 parts mass corncob, 10-20 parts mass bagasse, 15-25 parts mass bran, 3-8 parts mass corn flour, 20-30 parts mass calcium sulfate, 5-10 parts mass magnesium sulfate, 5-10 parts mass lime, 0.25-4 parts mass gypsum, 5-10 parts mass urea comprising 46% nitrogen, 5-10 parts mass triacontanol and 2-7 parts mass soybean meal. The mixed culture composition has moisture content of 65 plus minus 1% and pH of 7.5-9. The nutritional inorganic fertilizer comprises boron fertilizer and potassium fertilizer in a mass ratio of 1:1.5. The mixed culture composition is prepared by (a) mixing nutritional inorganic fertilizer, sawdust, oat hulls, litter, peanut shell, cottonseed hulls, calcium carbonate, corncob, bagasse, bran and corn flour, drying obtained mixture, and stirring dried mixture at 150-200 revolutions/minute (rpm) for 5 minutes, and (b) adding suitable amount of water to the mixture, stirring mixture for at least 15 minutes, sequentially adding the calcium carbonate, corncob, bagasse, bran, corn flour, calcium sulfate, magnesium sulfate, lime, gypsum, urea comprising 46% nitrogen, triacontanol and soybean meal, stirring the mixture at 200-210 rpm, controlling water content of the mixture reaches 65 plus minus 1%, and regulating pH of the mixture to 7.5-9.