NOVELTY - Extracting copper from waste printed circuit board comprises (i) crushing and grinding dismantled electronic component of the waste printed circuit board (PCB) to a powder; (ii) respectively carrying out copper(II)-resistant domesticated culturing on Acidithiobacillus ferrooxidans and Acidithiobacillus thiooxidans; (iii) mixing Acidithiobacillus ferrooxidans and Acidithiobacillus thiooxidans and culturing; (iv) adding waste PCB powder and reacting; and (v) extracting the leachate with a copper extraction agent.    USE - The method is useful for extracting copper from waste printed circuit board (claimed).    ADVANTAGE - The method realizes biological extraction, saves energy sources, and improves extraction rate of copper on the basis of improving the adding amount of the waste PCB powder.    DETAILED DESCRIPTION - Extracting copper from waste printed circuit board comprises (i) crushing and grinding dismantled electronic component of the waste printed circuit board (PCB) to a powder, screening the ground PCB waste powder and collecting the sieved waste PCB powder; (ii) respectively carrying out copper(II)-resistant domesticated culturing on Acidithiobacillus ferrooxidans and Acidithiobacillus thiooxidans to obtain 100-200 mmol/l copper(II) resistant Acidithiobacillus ferrooxidans and Acidithiobacillus thiooxidans, where the domesticated culturing of Acidithiobacillus ferrooxidans comprises extracting sewage sludge from the municipal sewage treatment plant aeration tank, enrichment culturing, isolating and purifying to obtain Acidithiobacillus ferrooxidans, culturing Acidithiobacillus ferrooxidans in 9K culture medium to log phase, inoculating the bacteria to 10 mmol/l copper(II) concentration culture medium according to inoculation amount of 10-20 vol.%, culturing to log phase, inoculating the bacteria to 20 mmol/l copper(II) concentration culture medium according to inoculation amount of 10-20 vol.%, repeating the above operation for each additional domesticated strain, where the culture medium used is increased with 10 mmol/l copper(II) concentration compared to the previous generation and obtaining 100-200 mmol/l copper(II) environment grown log phase Acidithiobacillus ferrooxidans, and where the domesticated culturing of Acidithiobacillus thiooxidans comprises extracting sewage sludge from the municipal sewage treatment plant aeration tank, enrichment culturing, isolating and purifying to obtain Acidithiobacillus thiooxidans, culturing Acidithiobacillus thiooxidans in culture medium to log phase, inoculating the bacteria to 10 mmol/l copper(II) concentration culture medium according to inoculation amount of 10-20 vol.%, culturing to log phase, inoculating the bacteria to 20 mmol/l copper(II) concentration culture medium according to inoculation amount of 10-20 vol.%, repeating the above operation for each additional domesticated strain, where the culture medium used is increased with 10 mmol/l copper(II) concentration compared to the previous generation and obtaining 100-200 mmol/l copper(II) environment grown log phase Acidithiobacillus thiooxidans; (iii) mixing Acidithiobacillus ferrooxidans and Acidithiobacillus thiooxidans according to the inoculating amount ratio of 1:1.5-2.5 to obtain mixed bacterial culture liquid and carrying out mixed bacterial culturing, where the initial culture pH is 1.5-1.8 and the mixed bacterial inoculum amount is 10-20% volume of the mixed bacterial culture liquid; (iv) culturing mixed bacterial culture liquid for 40-55 hours, then adding waste PCB powder, where the waste PCB powder is added into the mixed bacteria culture system for every 20-25 hours, prior to joining of 1-3 g/l PCB powder, increasing to 1l mixed bacteria culture system, where the total mass of the added waste PCB powder is 28-36 g, culturing for 175-200 hours, leaching the mixed bacterial reaction, centrifuging the reaction mixture and taking upper layer leachate; and (v) extracting the leachate with a copper extraction agent to obtain copper-containing extract, stripping with a stripping agent, introducing the resulting copper stripping solution into an electrolytic cell, electrowinning to obtain copper, and recovering copper.