NOVELTY - Method for domestication and screening of heterotrophic nitrification and aerobic denitrification bacteria, involves inoculating sample into nitrogen-enriched nutritional solution, performing culturing process, adding the wastewater to reactor, performing culturing process under aerobic conditions for rapid proliferation of bacteria, adding the wastewater to sequencing batch reactor (SBR) for domestication of bacteria, performing A/O process, purifying bacteria, and identifying bacteria having heterotrophic nitrification and aerobic denitrification properties.    USE - The method is useful for domestication and screening of heterotrophic nitrification and aerobic denitrification bacteria (claimed).    ADVANTAGE - The method screens bacteria which is tolerant to wastewater containing phenol, amine, heterocyclic, cyanogens or polycyclic aromatic hydrocarbon. The bacterium removes ammoniacal nitrogen and total nitrogen in water.    DETAILED DESCRIPTION - Method for domestication and screening of heterotrophic nitrification and aerobic denitrification bacteria, involves (a) inoculating sample into nitrogen-enriched nutritional solution comprising nitrogen-containing wastewater, and performing culturing process under shaking condition, (b) adding the wastewater to reactor, and performing culturing process under aerobic conditions for rapid proliferation of bacteria, (c) adding the wastewater to sequencing batch reactor (SBR) for domestication of heterotrophic nitrification and aerobic denitrification bacteria, (d) performing A/O process to obtain sludge containing heterotrophic nitrification and aerobic denitrification bacteria, (e) inoculating the sludge containing bacteria into filter medium, and purifying the heterotrophic nitrification and aerobic denitrification bacteria, (f) testing nitrification and denitrification proficiency of purified heterotrophic nitrification and aerobic denitrification bacteria, and (g) identifying bacteria having heterotrophic nitrification and aerobic denitrification properties.