NOVELTY - Evaluating ecological health of microbial community involves analyzing water quality and evaluation of microorganisms in a certain area. The pollutant concentration gradient is set with sampling points, each water sample is produced by high-throughput sequencing using gene chip. All water samples are analyzed with their quality to obtain each sample quantitative PCR or high throughput sequencing results. Microorganisms in each sample is calculated, where the relative abundance of a particular microorganism percentage of all microorganisms in the sample.    USE - Method for evaluating ecological health of microbial community (claimed).    ADVANTAGE - Method enables to evaluate ecological health of microbial community is simple, fast, healthy and eco-friendly manner.    DETAILED DESCRIPTION - Evaluating ecological health of microbial community involves analyzing water quality and evaluation of microorganisms in a certain area. The pollutant concentration gradient is set with sampling points, each water sample is produced by high-throughput sequencing using gene chip. All water samples are analyzed with their quality to obtain each sample quantitative PCR or high throughput sequencing results. Microorganisms in each sample is calculated, where the relative abundance of a particular microorganism percentage of all microorganisms in the sample. The calculated water quality index and relative abundance of microbial correlation coefficient values caarying out relative analysis. The correlation coefficient is obtained from microorganisms water quality index for all samples and corresponding samples and significance index value. The screening of specific water quality objectives related to the microbial population in the index, set a significant value of the standard. The obtained value to identify microbial species, which is associated with a corresponding water quality index of the microbial population. The screened positive microorganisms associated with the water quality index has a negative correlation with water quality index. The definition of a positive correlation between microbial species and negative microorganism species to perform the opposite function of the microbial population. The establishment of total microbial population in contact with relative abundance of specific water quality objectives for each sample is calculated. All water sample quality index is a positive correlation with relative abundance species of microorganisms. The negatively correlated microorganisms with relative abundance to value all samples of the water quality index. The water sample establishing positive and negative total relative abundance of two kind function microorganism series group with water quality index. The ecological health range and evaluation criteria to determine the value of a water quality index of all samples. The ecological health range and evaluation criteria graph is plotted, then S-shaped curve graph is obtained. The obtained graph is similar to logistic growth curve of association.