NOVELTY - Separation and purification of mangiferin from Aquilaria sinensis leaves involves drying A.sinensis leaves, pulverizing, percolating coarse powder, concentrating, extracting with petroleum ether and n-butanol, concentrating, performing column chromatography, distilling solvent to obtain mangiferin yellow powder, preparing ethyl acetate-methanol-water solvent system, leaving still where upper layer is mobile phase and lower layer is yellow powder, performing high-speed countercurrent chromatography, fractions containing mangiferin and distilling solvent.    USE - Separation and purification of mangiferin from A.sinensis leaves (claimed).    ADVANTAGE - The method is environmentally-friendly, and enables efficient and economical separation and purification of mangiferin with high quality and high purity, by simple method. The cost of the solvent and solvent treatment is reduced.    DETAILED DESCRIPTION - Separation and purification of mangiferin from Aquilaria sinensis leaves involves drying A.sinensis leaves, pulverizing the dried leaves, percolating or extracting the coarse powder, combining all the percolating fluids or extracts, concentrating by remove the organic solvent, extracting 2-4 times with petroleum ether to remove lipids and fat-soluble dye, extracting aqueous phase with n-butanol 2-4 times, combining n-butanol fractions, concentrating at 40-60 degrees C under low pressure to obtain extract containing mangiferin, performing normal-phase column chromatography by mixing extract with n-butanol, collecting the eluent, recovering solvent at 35-45 degrees C under low pressure to obtain extract containing mangiferin, combining extracts, distilling solvent under low pressure to obtain mangiferin yellow powder, preparing ethyl acetate-methanol-water solvent system, leaving still where upper layer is mobile phase and lower layer is yellow powder, performing high-speed counter-current chromatography, fractions containing mangiferin and distilling solvent.