NOVELTY - Generating sugars from biomass, comprises (a) providing a mixture comprising biomass, a non-ionic organic polymer and enzymes to hydrolyze components of the biomass to sugars, (b) incubating the mixture under conditions such that the enzymes hydrolyze components of the biomass to sugars, (c) separating the mixture into a liquid stream (5) and a solids stream (6), (d) separating the liquid stream with the filter into a permeate (8) and a retentate (7), and (e) returning the retentate to the mixture or a mixture comprising biomass for generating sugars and re-using the polymer.    USE - The method is useful for generating sugars, preferably glucose and xylose from biomass such as a lignocellulosic biomass and a pretreated biomass (all claimed), where the sugars are useful in the production of ethanol, biofuels, biochemicals and other chemical products.    ADVANTAGE - The method ensures cost-effective generation of sugars with increased yield at high conversion rate by reducing the amount of saccharification enzymes.    DETAILED DESCRIPTION - Generating sugars from biomass, comprises (a) providing a mixture comprising the biomass, a non-ionic organic polymer of sufficient size to be captured by a filter and enzymes to hydrolyze components of the biomass to sugars, (b) incubating the mixture under conditions such that the enzymes hydrolyze components of the biomass to sugars for producing a mixture (4) of solids and a liquid comprising the polymer and sugars, (c) separating the mixture into a liquid stream (5) comprising the polymer and sugars and a solids stream (6) comprising solids, (d) separating the liquid stream with the filter into a permeate (8) comprising sugars and a retentate (7) comprising the polymer, and (e) returning at least a portion of the retentate to the mixture or a mixture comprising biomass for generating sugars and re-using the polymer.    DESCRIPTION OF DRAWING(S) - The diagram shows a schematic view of a method for generating sugar.    Mixture of solid and liquid (4)    Solid stream (5)    Liquid stream (6)    Retentate (7)    Permeate. (8)