NOVELTY - Cultivation substrate comprises wooden meal, wheat bran, grape leaves, cornmeal, bamboo leaves powder, duckweed grass powder, Lysimachia powder, gypsum powder, glucose, urea, calcium superphosphate, amino acid composition, frankincense, ginseng leaves, herba violae, maple leaf grass, vermiculite, powdered activated carbon, clay, and nutritional additive.    USE - As cultivation substrate of Agaricus subrufescens (claimed).    ADVANTAGE - The substrate completely utilizes raw materials, has good carbon-nitrogen ratio, and produces healthy A.subrufescens with big head.    DETAILED DESCRIPTION - Cultivation substrate comprises 80-85 pts. wt. wooden meal, 20-22 pts. wt. wheat bran, 18-20 pts. wt. grape leaves, 18-20 pts. wt. cornmeal, 10-11 pts. wt. bamboo leaves powder, 8-10 pts. wt. duckweed grass powder, 6-7 pts. wt. Lysimachia powder, 1-2 pts. wt. gypsum powder, 2-3 pts. wt. glucose, 1-2 pts. wt. urea, 0.4-5 pts. wt. calcium superphosphate, 0.1-0.2 pts. wt. amino acid composition, 3-4 pts. wt. frankincense, 5-6 pts. wt. ginseng leaves, 2-3 pts. wt. herba violae, 3-4 pts. wt. maple leaf grass, 22-24 pts. wt. vermiculite, 3-4 pts. wt. powdered activated carbon, 20-21 pts. wt. clay, and 8-10 pts. wt. nutritional additive. The nutritional additive comprises 0.1-0.2 pts. wt. Coix seed oil, 15-18 pts. wt. diatomite, and 30-32 pts. wt. vinegar. The nutritional additive is prepared by mixing the vinegar and the Coix seed oil, soaking diatomite in mixture for 15-20 minutes, drying soaked product, and crushing dried product. An INDEPENDENT CLAIM is included for method for preparing the cultivation substrate, which involves (a) dissolving glucose, calcium superphosphate and urea in 10-12 times water, (b) mixing vermiculite, powdered activated carbon and clay, adding water to mixture until water content becomes 28-30%, pressing mixture into rod, roasting the rod at 500 degrees C for 2 hours, (c) drying and crushing grape leaves, frankincense, ginseng leaves, herba violae and maple leaf grass, along with the rod, and adding water to crushed product until water content becomes 65-68%, and (d) mixing products of steps (a), (b) and (c), sealing mixture in polyethylene plastic bag, and sterilizing the bag using steam at 100 degrees C under normal pressure to obtain finished product.