NOVELTY - Efficient extraction and separation of bulleyaconitine includes extracting aconitum herbs with ethanol, concentrating, adding sulfuric acid and aqueous ammonia, pouring into separatory funnel, extracting with ethyl acetate, concentrating, adding purified water, sulfuric acid and aqueous ammonia, precipitating, and collecting aconitum alkaloid precipitate; dissolving in ethyl acetate, mixing with silica, eluting through silica gel column chromatography, monitoring, and collecting bulleyaconitine-containing eluent fractions; and concentrating, mixing with ethyl acetate, and precipitating.    USE - Method for efficient extraction and separation of bulleyaconitine (claimed).    ADVANTAGE - The method is environment-friendly, saves energy, uses nontoxic or low-toxicity organic solvent, achieves bulleyaconitine highest of up to 98-99% and high purity, and it has better separation effect, zero solvent residue, increased extraction rate of up to 1.5-2.2%, and reduced organic solvent type and dosage.    DETAILED DESCRIPTION - Efficient extraction and separation of bulleyaconitine comprises:    (A) removing impurities of aconitum herbs, crushing, adding into round bottom flask, and adding 3.2 times 3% sulfuric acid; extracting with 75 wt.% ethanol by controlling steam pressure to 0.1 MPa and heating to 60 degrees C for 12 hours, filtering, and collecting filtrate; repeating above extraction steps twice by adding residue with 2.4 times 3% sulfuric acid, extracting with 75 wt.% ethanol for 2-4 hours, filtering, and collecting filtrates; and combining filtrates, vacuum-concentrating to 0.02-0.04 MPa until no more alcohol taste, and diluting concentrate with 1.5 times purified water until relative density is 1.05-1.07;    (B) adding 10 wt.% sulfuric acid to adjust pH to 2, standing for 24 hours, filtering, adding filtrate with concentrated aqueous ammonia while stirring to adjust pH to 8, standing for 24 hours, filtering, and collecting filtrate;    (C) pouring into separatory funnel, adding 1.6-2 times ethyl acetate to carry out extraction while stirring for 30-45 minutes, standing for 2-3 hours, separating ethyl acetate extract upper layer, repeating extraction step on aqueous liquid lower layer for 3 times, and combining ethyl acetate extracts; vacuum-concentrating to 0.02-0.04 MPa while controlling temperature to 60 plus minus 5 degrees C, recovering ethyl acetate to obtain thick paste, adding 10 times purified water, mixing, adding 10 wt.% sulfuric acid while stirring to adjust pH to 2, standing for 12 hours, filtering, slowly adding aqueous ammonia to adjust pH to 8.5-9, standing for 12 hours, filtering, precipitating with purified water until pH is neutral, and collecting aconitum alkaloid precipitate; and dissolving in ethyl acetate, mixing with 0.6-1.2 times silica, drying until moisture content is less than 15%, placing into clean container, and sealing;    (D) eluting through silica gel column chromatography using petroleum ether, ethyl acetate and triethylamine at volume ratio of 4:1:0.075 as solvent system, controlling flow rate to 13-20 mL/minute, monitoring through thin layer chromatography, collecting bulleyaconitine-containing eluent fractions, and combining; and    (E) vacuum-concentrating to 60 plus minus 5 degrees C at 0.02-0.04 MPa through rotary evaporator to obtain bulleyaconitine crude crystals, adding into round-bottomed flask, mixing with 4 times ethyl acetate to dissolve crude crystals, vacuum-concentrating to 1/3 volume at 0.02-0.04 MPa, precipitating with petroleum ether by shaking, standing to completely crystallize, filtering, washing with petroleum ether, and drying.