NOVELTY - A straight-through amoxicillin preparation process is performed by taking 6-10% penicillin degreasing liquid, adding penicillin acylase in air-tight reactor, controlling pH to 8-8.5, reacting at 28-37 degrees C, detecting penicillin content, computing penicillin conversion rate to be greater than or equal to 97% to obtain release material liquid, intercepting penicillin acylase in bottom sieve of reactor to obtain filtrate, transferring filtrate to nanofiltration system, concentrating to obtain 70-120 g/L 6-aminopenicillanic acid (6-APA) solution, adding 18-30% hydrochloric acid aqueous solution, and adjusting pH to 1-2.    USE - A straight-through amoxicillin preparation process (claimed).    ADVANTAGE - The method reduces operational processes and labor intensity, is low cost, environment-friendly, and safe.    DETAILED DESCRIPTION - A straight-through amoxicillin preparation process is performed by taking 6-10% penicillin degreasing liquid, adding penicillin acylase in air-tight reactor, controlling pH to 8-8.5, reacting at 28-37 degrees C, detecting penicillin content, computing conversion rate of penicillin to be greater than or equal to 97% to obtain release material liquid, intercepting penicillin acylase in bottom sieve of reactor to obtain filtrate, transferring filtrate to nanofiltration system, concentrating to obtain 70-120 g/L 6-APA solution, adding 18-30% hydrochloric acid aqueous solution, adjusting pH to 1-2, adding 50-70 vol.% butanol and/or butyl ester, separating water phase, passing through macroporous resin column, collecting 6-APA effluent, dropping ammonia solution, adjusting pH of effluent to 6-6.5, detecting phenylacetic acid content in effluent to be less than or equal to 100 ppm based on detection result of terminating column, adding 6-APA effluent in reactor containing penicillin acylase, adding hydroxy phenyl glycine methyl ester at molar ratio hydroxy phenyl glycine methyl ester:6-APA of (1-1.1):1, stirring, dropping ammonia solution, adjusting pH to 6.3-6.5, controlling temperature at 16-18 degrees C, detecting 6-APA content, and conversion rate to be greater than or equal to 99%, intercepting penicillin acylase in bottom sieve of reactor to obtain filtrate, dissolving filtrate in tank, purifying by washing with 1/3 amount of water, combining washing liquid to filtrate to obtain suspension, cooling at 5-10 degrees C, dropping 8-12% hydrochloric acid solution, filtering, transferring filtrate in crystallizer, crystallizing at 5-10 degrees C, dropping 2.7-3.3 mol/L ammonia solution, separating solid in filtrate, stopping dropping of ammonia solution, continuously stirring for 20-40 minutes, continuously dropping 2.7-3.3 mol/L ammonia solution until pH is 5.1, stopping dropping of ammonia solution, continuously stirring for 3-5 hours, filtering, repeatedly washing to remove salt, washing filter cake with acetone, and drying.