NOVELTY - Preparation of plerixafor involves dissolving 1,4,8,11-tetraaza-5,7,12-trioxocyclotetradecane in dry acetonitrile to obtain first homogeneous solution, dissolving terephthalic acid dichloride in triethylamine to obtain second homogeneous solution, performing coupling reaction in conventional reactor or microreactor to obtain white solid, reacting white solid with sodium borohydride and glacial acetic acid in dioxane, regulating pH, recovering solvent, adding distilled water, stirring, regulating pH, vacuum-distilling solvent, extracting, vacuum-concentrating and recrystallizing.    USE - Method for preparing plerixafor (claimed).    ADVANTAGE - The green environment-protecting method has plerixafor yield of 81.8%, and 1,4,8,11-tetraaza-5,7,12-trioxocyclotetradecane yield of 99.2%. It has high terephthalic acid dichloride activity, high production efficiency and easy implementation for industrial production. It uses simple process device.    DETAILED DESCRIPTION - Preparation of plerixafor comprises:    (A) dissolving 1,4,8,11-tetraaza-5,7,12-trioxocyclotetradecane in dry acetonitrile to obtain first homogeneous solution, and dissolving terephthalic acid dichloride in triethylamine to obtain second homogeneous solution;    (B) performing coupling reaction of first and second homogeneous solutions in conventional reactor by mixing in bottle, reacting, filtering and recrystallizing with dimethyl sulfoxide/ethanol mixture to obtain white solid, or performing coupling reaction of first and second homogeneous solutions in microreactor by simultaneously injecting solutions in microreactor with measuring pump, mixing, reacting, collecting effluent, filtering, drying filter cake and recrystallizing with dimethyl sulfoxide/ethanol mixture to obtain white solid; and    (C) reacting white solid with sodium borohydride and glacial acetic acid in dioxane for 40-50 hours, regulating pH at 1-3 using hydrochloric acid solution, recovering solvent, adding distilled water, stirring to dissolve, regulating pH at 11-13 using sodium hydroxide solution, vacuum-distilling solvent, extracting with dichloromethane, vacuum-concentrating and recrystallizing with ethanol/acetone mixture.    DESCRIPTION OF DRAWING(S) - The drawing shows a process flow diagram of preparation of plerixafor (Drawing includes non-English language text).