NOVELTY - Micro-fluidic chip is double layered integrated chip with 12 sets of parallel micro-fluidic unit arranged in parallel manner. Each micro-fluidic unit has small diameter hole with fluid passage. The micro-fluidic chip is prepared by using photolithography technology, which contains SU-8 polymer template that has multiple apertures having small diameter. The SU-8 polymer template is heated and molded to rectangular-shaped orifice having hole. The SU-8 polymer template is heated under ultra-violet radiations exposure.    USE - Micro-fluidic chip for screening diameter of three-dimensional U87 cells and anti-cancer drugs (claimed).    ADVANTAGE - The micro-fluidic chip is simple, eco-friendly, fast and cost-effective as it does not require organic reagents.    DETAILED DESCRIPTION - Micro-fluidic chip is double layered integrated chip with 12 sets of parallel micro-fluidic unit arranged in parallel manner. Each micro-fluidic unit has small diameter hole with fluid passage. The micro-fluidic chip is prepared by using photolithography technology, which contains SU-8 polymer template that has multiple apertures having small diameter. The SU-8 polymer template is heated and molded to rectangular-shaped orifice having hole. The SU-8 polymer template is heated under ultra-violet radiations exposure. The SU-8 polymer template is mixed with polydimethylsiloxane (PDMS) uncured polymer solution, which is cured by heating. The cured PDMS solution is stripped to obtain chip, which is molded on counter-mold chip to obtain PDMS polymer. The PDMS uncured polymer solution is poured onto PDMS polymer on counter-mold chip and then stripped to obtain cured PDMS polymer chip with hole having small diameter. Soft lithography technology is used to produce fluid passage in PDMS polymer chip. Plasma instrument is used to prepare irreversible PDMS polymer chip containing fluid passage and different diameter hole. Surface of PDMS chip is modified by treating with 0.2% Pluronic (RTM: nonionic, surfactant polyol)F-127 for 4 hours. The treated chip is washed (2 times) with sterile water and phosphate buffered saline to obtain micro-fluidic chip.