NOVELTY - (NE,E)-4-((1E,3E)-4-(((2S)-4-carbamoyl-1-carboxybutan-2-yl)carbamoyl)buta-1,3-dien-1-yl)-N-((4-((1E,3E)-4-(((2S)-4-carbamoyl-1-carboxybutan-2-yl)carbamoyl)buta-1,3-dien-1-yl)phenyl)imino)benzen-1-imine oxide, (NE,E)-4-((1E,3E)-4-(((2S)-4-carbamoyl-1-carboxybutan-2-yl)carbamoyl)buta-1,3-dien-1-yl)-N-((4-((1E,3E)-4-carboxybuta-1,3-dien-1-yl)phenyl)imino)benzen-1-imine oxide and (NE,E)-4-((1E,3E)-4-carboxybuta-1,3-dien-1-yl)-N-((4-((1E,3E)-4-carboxybuta-1,3-dien-1-yl)phenyl)imino)benzen-1-imine oxide hydrate (I-III), are new.    USE - Compound of formulae (I-III) used for inhibiting the activity of protein tyrosine phosphatase 1B (claimed) for treating type 2 diabetes.    ADVANTAGE - The compound of formulae (I-III) are cost-effective, environmental friendly and are prepared in a simple way.    DETAILED DESCRIPTION - (NE,E)-4-((1E,3E)-4-(((2S)-4-carbamoyl-1-carboxybutan-2-yl)carbamoyl)buta-1,3-dien-1-yl)-N-((4-((1E,3E)-4-(((2S)-4-carbamoyl-1-carboxybutan-2-yl)carbamoyl)buta-1,3-dien-1-yl)phenyl)imino)benzen-1-imine oxide, (NE,E)-4-((1E,3E)-4-(((2S)-4-carbamoyl-1-carboxybutan-2-yl)carbamoyl)buta-1,3-dien-1-yl)-N-((4-((1E,3E)-4-carboxybuta-1,3-dien-1-yl)phenyl)imino)benzen-1-imine oxide and (NE,E)-4-((1E,3E)-4-carboxybuta-1,3-dien-1-yl)-N-((4-((1E,3E)-4-carboxybuta-1,3-dien-1-yl)phenyl)imino)benzen-1-imine oxide hydrate of formulae (I-III) are new. An INDEPENDENT CLAIM is included for a method for preparing compound, which involves:    (A) inoculating Chattanooga Streptomyces L10 spores (10 mu g) in plate medium (30mL), and culturing at 28 degrees C for 7-8 days with sterile water (5mL) to obtain spore suspension, where the plate medium comprises water as solvent, yeast extract (0.4 wt.%), malt extract (1 wt.%), glucose (0.4 wt.%), calcium carbonate (0.2 wt.%) and agar powder (2 wt.%) at a pH of 6.8-7.3, and the Chattanooga Streptomyces strain L10 is preserved at China General Microbiological Culture Collection Center (CGMCC No: 2644);    (B) inoculating the obtained spore suspension (0.1mL) into the seed medium (10mL), adding glass beads (5) having diameter of 6mm, and placing on a shaker at 28 degrees C for 48 hours at a speed of 200 rotations per minute (rpm) to obtain seed culture broth, where the seed medium comprises water as solvent, glucose (1.75 wt.%), peptone (1.5 wt.%) and sodium chloride (1 wt.%) at a pH of 6.8-7.3;    (C) inoculating the obtained seed culture (1mL) in the fermentation medium (100mL), adding glass beads (10) having diameter of 6mm, and placing on a shaker at 28 degrees C for 4 days at a speed of 200rpm to obtain fermentation broth, where the fermentation medium comprises water as solvent, yeast extract (0.3 wt.%), malt extract (0.3 wt.%), tryptone (0.5 wt.%), and glucose (1 wt.%) at a pH of 6.8-7.3;    (D) mixing the obtained fermentation broth with ethanol in the volume ratio of 1:1, concentrating by distillation under reduced pressure to a volume of 1/10 of the fermentation broth, adjusting pH to 10.2, and centrifuging to obtain supernatant;    (E) adjusting the pH of the supernatant to 2, discarding the centrifugal supernatant, dissolving the precipitate portion in dimethyl sulfoxide, and performing separation by semi-preparative high performance liquid chromatography, where the agilent ZORBAX SB-C18 is used for column, mobile phase A is aqueous solution of trifluoroacetic acid ( 0.1%), mobile phase B is TFA (0.1%) in methanol, flow rate is 3mL/minute and B phase is raised from 10-100% for 0-30 minutes at gradient conditions; and    (F) detecting the separated compounds at a wavelength of 400nm, where the compound of formula (I) is obtained in a retention time of 20-21 minutes, compound of formula (II) is obtained in a retention time of 23-24 minutes, and compound of formula (III) is obtained in a retention time of 27-28 minutes.