NOVELTY - Real time fluorescence quantitative PCR method for detecting apple stem pox virus involves using infected cDNA as template, and ASPV-cp-FjP ASPV-cp-2 as primer for PCR amplification, obtaining amplified product, performing agarose gel electrophoresis, transformation process, and selecting positive clone, performing plasmid extraction, and gradient dilution, using positive recombinant plasmid as template and ASPV-probe3-F and ASPV-probe3-R as primer constructing standard curve, performing real time fluorescence quantitative PCR, and comparing with standard curve.    USE - The real time fluorescence quantitative PCR method is useful for detecting apple stem pox virus (claimed).    ADVANTAGE - The real time fluorescence quantitative PCR method enables to detect apple stem pox virus which avoids false positive result and environment pollution problem.    DETAILED DESCRIPTION - Real time fluorescence quantitative PCR method for detecting apple stem pox virus involves using infected cDNA as template, and ASPV-cp-FjP ASPV-cp-2 as primer for PCR amplification, obtaining amplified product, performing agarose gel electrophoresis to detect amplified product, performing transformation process, and selecting positive clone, performing plasmid extraction and identifying standard recombinant plasmid, performing gradient dilution of positive recombinant plasmid as standard, using positive recombinant plasmid as template and ASPV-probe3-F and ASPV=probe3-R as primer constructing standard curve, performing real time fluorescence quantitative PCR using test material for cDNA as a template, and comparing result with standard curve. The ASPV-Cp-F2 comprises base pair sequence of SEQ ID NO: 1. The ASPV-Cp-R2 comprises base pair sequence of SEQ ID NO: 2. The ASPV-Probe3-F comprises base pair sequence of SEQ ID NO: 3. The ASPV-Probe3-R comprises base pair sequence of SEQ ID NO: 4. The ASPV-Probe3 comprises base pair sequence of SEQ ID NO: 5. cccattaggttagggtgtagttgct (SEQ ID NO: 1), atgaaagaacacacacatagccgc (SEQ ID NO: 2), agcagaccgaggggtgtactct (SEQ ID NO: 3), gcggtacatctgtatttccttgct (SEQ ID NO: 4), and ttgtgccttctacgcgaagcatgtc (SEQ ID NO: 5).