NOVELTY - Preparing diesel oil degrading bacterial immobilized particles, comprises (i) drying carrier shell Artemia cysts, and grinding; (ii) culturing Arthrobacter strain; (iii) centrifuging adsorbed materials under sterile conditions, adding 0.5-1.5 g halogen egg shell carrier/100 ml sodium alginate, adding sodium alginate solution into Artemia cysts shell carrier, mixing, adding 500-800 ml calcium chloride solution/100 ml sodium alginate solution, adding calcium chloride solution into sodium alginate, and cross-linking; and (iv) washing, packaging, and preserving.    USE - The method is useful for preparing diesel oil degrading bacterial immobilized particles (claimed).    ADVANTAGE - The method has simple operation, is environmental-friendly, and produces product having degradation of diesel oil capacity reaching up to more than 65%.    DETAILED DESCRIPTION - Preparing diesel oil degrading bacterial immobilized particles, comprises (i) drying carrier shell Artemia cysts at 50-70 degrees C by placing in a dry box, grinding in a grinder, and filtering out particle diameter of 0.1-0.5 mm particles, and autoclaving; (ii) culturing Arthrobacter strain in a lysogeny-broth (LB) culture medium, adding 0.5-1.5 g halide shell eggs/100 ml Arthrobacter strain, mixing Artemia cysts with Arthrobacter strain at 100-130 rotations/minute, culturing the absorbed materials for 4-10 hours at 25-37 degrees C, to obtain bacterial strain completely absorbed to shell of Artemia cysts carrier; (iii) centrifuging the adsorbed materials under sterile conditions at a speed of 6000-12000 rotations/minute at 2-10 minutes, discarding the supernatant, adding 0.5-1.5 g halogen egg shell carrier/100 ml sodium alginate, adding 2-3% sodium alginate solution into Artemia cysts shell carrier, mixing, adding 500-800 ml calcium chloride solution/100 ml sodium alginate solution, pipetting solution, adding 4-5% calcium chloride solution dropwise into sodium alginate with constant stirring, and cross-linking for 20-50 minutes, to obtain immobilized particles of 3-5 mm; and (iv) washing the immobilized particles with sterile double distilled water for 2-4 times, washing surface strain on immobilized particles, and packaging, to obtain the final product, and preserving at 4 degrees C.