NOVELTY - Cultivation substrate comprises 40-45 pts. wt. mulberry branch meal, 25-28 pts. wt. cotton seed hull, 18-22 pts. wt. cypress, 16-18 pts. wt. oatmeal, 13-15 pts. wt. sweet potato seedling powder, 10-12 pts. wt. peanut seedling powder, 8-10 pts. wt. maggot powder, 9-11 pts. wt. mung bean flour, 12-15 pts. wt. diatomite, 7-9 pts. wt. mussel meat powder, 6-7 pts. wt. horsetail, 3-4 pts. wt. sedges, 4-5 pts. wt. dandelion, and 3-4 pts. wt. nutritional additive.    USE - As cultivation substrate of Hypsizygus ulmarius (claimed).    ADVANTAGE - The substrate is cost-effective, and utilizes waste materials.    DETAILED DESCRIPTION - Cultivation substrate comprises 40-45 pts. wt. mulberry branch meal, 25-28 pts. wt. cotton seed hull, 18-22 pts. wt. cypress, 16-18 pts. wt. oatmeal, 13-15 pts. wt. sweet potato seedling powder, 10-12 pts. wt. peanut seedling powder, 8-10 pts. wt. maggot powder, 9-11 pts. wt. mung bean flour, 12-15 pts. wt. diatomite, 7-9 pts. wt. mussel meat powder, 6-7 pts. wt. horsetail, 3-4 pts. wt. sedges, 4-5 pts. wt. dandelion, and 3-4 pts. wt. nutritional additive. The nutritional additive comprises 1-1.2 pts. wt. zinc gluconate, 0.1-10.15 pts. wt.%. Coix seed oil, 5-7 pts. wt. zeolite powder, 1-2 pts. wt. bioslurry, 3-4 pts. wt. white wine, 8-10 pts. wt. wheat flour, 3-4 pts. wt. southernwood, and 2-3 pts. wt. velvet grass. The nutritional additive is prepared by mixing zinc gluconate, Coix seed oil, zeolite powder, bioslurry, southernwood and velvet grass, drying mixture, crushing dried mixture, adding wheat flour and white wine to crushed product, and fermenting mixture for 20-22 hours to obtain product. An INDEPENDENT CLAIM is included for method for preparing the cultivation substrate, which involves (a) soaking mulberry branch meal and cotton seed hull in water for 20-24 hours, and extracting materials for 3-4 hours, (b) taking horsetail, sedges, cypress and dandelion, adding materials to product of step (a), stirring added materials for 25-30 minutes, and controlling moisture content of mixture at 60-65%, and (c) packing the mixture in bag, and sterilizing the bag using steam at 100 degrees C under normal pressure to obtain finished product.