NOVELTY - Preparation of high-purity 10-deacetylbaccatin III involves adding crushed Taxus media leaves to an extraction tank, adding alcohol solution, soaking for 8-12 hours, performing percolation extraction, collecting leachate, aging, removing supernatant, washing resultant precipitate with tap water, loading resultant liquid onto a macroporous resin column, adsorbing 10-deacetylbaccatin III using macroporous resin, eluting, concentrating the eluate, adding obtained crude product to acetonitrile, crystallizing, and recrystallizing obtained crystallized product.    USE - Preparation of high-purity 10-deacetylbaccatin III (claimed).    ADVANTAGE - The method enables economical preparation of high-purity 10-deacetylbaccatin III by simple and environmentally-friendly process.    DETAILED DESCRIPTION - Preparation of high-purity 10-deacetylbaccatin III involves adding crushed Taxus media leaves to an extraction tank, adding 40-60% alcohol solution, soaking for 8-12 hours, performing percolation extraction for 10-20 hours, collecting leachate, aging the leachate at 60-70 degrees C for 12-20 hours under vacuum degree of -0.06 MPa to -0.08 MPa to recover alcohol, removing supernatant, washing the resultant precipitate with tap water at 70-80 degrees C for 2-3 times, loading resultant liquid onto a macroporous resin column, adsorbing 10-deacetylbaccatin III using macroporous resin, washing macroporous resin column with tap water to remove impurities, eluting with 40-70% alcohol solution, concentrating the eluate at 60-70 degrees C under vacuum degree of -0.06 MPa to -0.08 MPa to dryness, adding acetone to obtained crude product (I), stirring, filtering, obtaining acetone solution and residue, dissolving residue in acetone, filtering, combining acetone solutions, concentrating at 70-80 degrees C, adding obtained dry product to dichloromethane, stirring, filtering, obtaining dichloromethane solution and residue, dissolving residue in dichloromethane, stirring, filtering, combining dichloromethane solutions, concentrating at 70-80 degrees C to recover dichloromethane, adding obtained crude product to acetonitrile, stirring, heating to 50-60 degrees C, naturally cooling for 12-24 hours, centrifuging, vacuum-drying, recrystallizing obtained crystallized product with ethanol and acetone, respectively for 2-3 times, and detecting crystals by liquid chromatography. The content ratio of crushed Taxus media leaves and alcohol solution is 1 kg:3-4 L. The purity of 10-deacetylbaccatin III is more than 98%.