NOVELTY - Cultivation of needle mushroom includes providing medicine residue, cottonseed hull, wheat bran and gypsum powder to prepare mushroom nutrient, preparing culture medium comprising potato, glucose, agar and water, adding the culture medium with vitamin B1 or vitamin B2, adding the mushroom nutrient and culture medium in sterilized bottle, inoculating parent species, culturing, placing in sterilized plastic bag, cooling to room temperature, placing the sterilized plastic bag containing the cultured mushroom in the inoculating bottle, putting in culturing room and culturing.    USE - Cultivation of needle mushroom (claimed).    ADVANTAGE - The method provides needle mushroom, which meets the GB7096-86 sanitary standards, thus, safe for human health.    DETAILED DESCRIPTION - Cultivation of needle mushroom comprises providing 13.5% medicine residue, 60% cottonseed hull, 25% wheat bran and 1.5% gypsum powder to prepare mushroom nutrient, preparing culture medium comprising 200 g potato, 20 g glucose, 20 g agar and 1000 ml water, adding the culture medium with vitamin B1 or vitamin B2, adding the mushroom nutrient and culture medium in 500 ml sterilized bottle, inoculating parent species at 5-6 pieces/bottle, culturing at 24 degrees C for 20 days, when mycelial growth is observed, culturing at 7-30 degrees C, preferably 23 degrees C, further growing at 3-18 degrees C, preferably 12-13 degrees C, placing in sterilized plastic bag, cooling to room temperature, taking an inoculating bottle, adding 10 ml formaldehyde and 5 g potassium permanganate/m3, sterilizing for 30 minutes, placing the sterilized plastic bag containing the cultured mushroom in the inoculating bottle at 25-30 bags/bottle, putting in culturing room, culturing for 24 hours at 20-25 degrees C, culturing at room temperature for 40-50 days, controlling the temperature at 6-8 degrees C and relative air humidity at 85-90% under very weak light illumination condition, and charging carbon dioxide at a concentration of more than 0.11-0.15%.