NOVELTY - A mycotoxin detection database is constructed by providing or configuring mycotoxin series standard which includes concentration gradient, detecting by enzyme-linked immunosorbent assay (ELISA), determining series of standards, performing linear regression analysis to obtain standard curve.    USE - Construction of mycotoxin detection database for mycotoxin detecting kit, detection of mycotoxin, and mycotoxin detecting device (all claimed).    ADVANTAGE - Construction process saves times and is fast and environment-friendly.    DETAILED DESCRIPTION - A mycotoxin detection database is constructed by providing or configuring N mycotoxin series standard which includes M concentration gradient, where N and M are natural numbers, detecting by enzyme-linked immunosorbent assay (ELISA), determining series of standards according to equation Nasterisk(summation of Rq+R), and performing linear regression analysis to obtain standard curve, where q is number of ELISA factors, Rq is q for 1st set of factors in variable, q, R, P are natural numbers, and factors of P and q are based according to the equation given in the document.    M= greater than or equal to 5; and    q= greater than or equal to 2.    INDEPENDENT CLAIMS are included for: (1) mycotoxin detecting kit comprising kit (a) which consists of mycotoxin and enzyme connecting plate, horseradish peroxidase-conjugated secondary antibody, mycotoxin monoclonal antibody or polyclonal antibodies, mycotoxin standard O solution (a) having mycotoxin concentration of 0 mu g/L and is serum albumin-containing phosphate buffer, chromogenic substrate solution (A) consisting of hydrogen peroxide or carbamide peroxide, chromogenic substrate solution (B) consisting of O-phenylenediamine, tetramethyl benzidine sulfate, or aminosalicylic acid, stop solution (a) which consists of hydrochloric acid buffer solution or buffer solution, cleaning liquid (a) having pH of 7-8 and consists of 0.1-0.5% Tween-20 (RTM: Polysorbate (20)) and 0.1-2% sodium azide phosphate buffer, antibody diluent (a) having pH of 7-8 containing 2-15% serum phosphate buffer, and complex solution (a) containing 0.5-2% fetal calf serum and 5-80% methanol containing phosphate buffer and kit (b) which consists of secondary mycotoxin and enzyme connecting plate, alkaline phosphatase-labeled mycotoxins antigen, mycotoxin monoclonal antibody or polyclonal antibody, O mycotoxin standard solution (b) having mycotoxin concentration of 0 mu g/L and is serum albumin-containing phosphate buffer, chromogenic substrate solution (b) consisting of nitro phosphate buffer, stop solution (b) consisting of sodium hydroxide buffer, washing solution (b) having pH of 7-8 and containing 0.5-2% Tween-20 and 0.1-0.5% sodium azide, and complex solution (b) containing 30-70% methanol and 0.5-2% serum phosphate buffer; (2) detection of mycotoxin which involves taking test sample mycotoxin, its pretreatment, and treatment kits, determining OD value by microplate reader and test sample, using invoke analysis module according to type of mycotoxins tested, inputting OD value, adjusting predetermined program, operating preset program, and reading result; and (3) mycotoxin detecting device.    DESCRIPTION OF DRAWING(S) - The drawing shows a process flow diagram of mycotoxin detection database. (Drawing includes non-English language text).