NOVELTY - Culture substrate comprises chestnut shell, chestnut leaves, Aloe, sawdust, wheat bran, beer lees, rapeseed meal, calcium superphosphate, sucrose, potassium dihydrogen phosphate, coconut shell activated carbon, methyl cellulose, laterite, bottle gourd seed powder, mint powder, herba epimedii, prepared root of Rehmannia, Prunus humilis seeds, Lygodium japonicum, and nutritional additive.    USE - The substrate is useful for cultivating Flammulina velutipes (claimed).    ADVANTAGE - The substrate cultivates F.velutipes exhibiting healthcare effect.    DETAILED DESCRIPTION - Culture substrate comprises 30-35 pts. wt. chestnut shell, 10-12 pts. wt. chestnut leaves, 8-10 pts. wt. Aloe, 65-70 pts. wt. sawdust, 20-22 pts. wt. wheat bran, 18-19 pts. wt. beer lees, 12-15 pts. wt. rapeseed meal, 0.3-0.4 pts. wt. calcium superphosphate, 3-4 pts. wt. sucrose, 0.3-0.5 pts. wt. potassium dihydrogen phosphate, 4-5 pts. wt. coconut shell activated carbon, 0.2-0.3 pts. wt. methyl cellulose, 25-26 pts. wt. laterite, 2-3 pts. wt. bottle gourd seed powder, 3-4 pts. wt. mint powder, 4-5 pts. wt. herba epimedii, 5-6 pts. wt. prepared root of Rehmannia, 3-4 pts. wt. Prunus humilis seeds, 4-5 pts. wt. Lygodium japonicum, and 5-6 pts. wt. nutritional additive. The nutritional additive comprises 12-14 pts. wt. hair, 2-3 pts. wt. furfural residue, 0.1-0.2 pts. wt. fingered citron oil, 3-4 pts. wt. zeolite powder, and 2-3 pts. wt. tea leaves. The additive is prepared by adding 6 mol/l hydrochloric acid solution to the hair in ratio of 1:1.7, hydrolyzing the mixture at 100-110 degrees C for 2 hours, adjusting the solution to neutral pH using sodium hydroxide solution, adding the remaining materials, heating the materials under reduced temperature, stirring the heated mixture, concentrating the stirred mixture, drying the concentrate, grinding the dried concentrate to obtain powder. An INDEPENDENT CLAIM is included for method for preparing the substrate which involves (a) mixing chestnut shell, chestnut leaves, Aloe, sawdust, wheat bran, beer lees, and rapeseed meal, adding 1-2% lime to the mixture, and stirring the mixture until the moisture content reaches 68-70%, (b) extracting herba epimedii, prepared root of Rehmannia, P.humilis seeds, and L.japonicum by adding 5-6 times water for 40-50 minutes, filtering the extract, stirring the filtrate under reduced temperature to obtain paste, mixing sucrose, and methyl cellulose, adding 6-7 times water, stirring the mixture, adding the paste to the mixture, pressing the mixture into rod shape, and drying the rod, and (c) placing the dried rod in a polyethylene bag, filling the product obtained in step (a) in the bag, and carrying out steam sterilization of the bag at 100 degrees C.