NOVELTY - Method for separating polysaccharide and protein involves adding distilled water to degreased fungi mycelium/sporocarp, refluxing, centrifuging, concentrating, dialyzing, drying, adding obtained fungus aqueous extract to aqueous two-phase system comprising choline chloride ionic liquid, potassium phosphate and distilled water, vibrating, centrifuging, separating, obtaining inorganic salt phase containing fungus polysaccharide, and ionic liquid phase containing protein and impurities, dialyzing inorganic salt phase, precipitating, centrifuging, and obtaining fungus polysaccharides.    USE - Method for separating polysaccharide and protein using choline ionic liquid two-phase aqueous system.    ADVANTAGE - The method enables economical and efficient separation of polysaccharide and protein by rapid and environmentally-friendly process.    DETAILED DESCRIPTION - Method for separating polysaccharide and protein involves degreasing crushed fungi mycelium or sporocarp with petroleum ether in Soxhlet extractor, refluxing at 30-60 degrees C for 6 hours, drying, adding distilled water to degreased fungi mycelium or sporocarp with solid-liquid ratio of 1: 10 g/mL, refluxing at 95 degrees C for 8 hours, centrifuging at 5000 rpm for 10 minutes, concentrating, dialyzing with distilled water for 48 hours, freeze-drying, formulating obtained fungus aqueous extract into 10 mg/mL aqueous solution of fungus aqueous extract, adding 1 mL aqueous solution of fungus aqueous extract to aqueous two-phase system, vibrating at 20 degrees C for 30 minutes in a water bath, centrifuging, performing two-phase separation, obtaining inorganic salt phase containing fungus polysaccharide as lower layer and ionic liquid phase containing protein and impurities as upper layer, dialyzing inorganic salt phase with distilled water for 48 hours, precipitating with 95% ethanol, aging at 4 degrees C overnight, centrifuging at 5000 rpm for 10 minutes, collecting the precipitate, freeze-drying, obtaining fungus polysaccharides, carrying out extraction on ionic liquid phase using dichloromethane, concentrating and recycling the ionic liquid. The aqueous two-phase system comprises choline chloride ionic liquid, potassium phosphate and distilled water in mass ratio of 0.25-0.5:0.7-1:1.