NOVELTY - Method of utilizing waste and hair of livestock (e.g. pig and cattle) to prepare peptone and application of peptone in fermentation of butyric acid bacteria, are claimed. The method involves immersing hair of livestock in tap water, washing the livestock hair, repeating step for 2-3 times, crushing dried livestock hair, mixing e.g. pig hair with mixed acid solution, carrying out acid hydrolysis, adding enzyme powder, carrying out e.g. enzymolysis and decolorization, carrying out filtration and clarification, and concentrating and spray-drying product.    USE - The method is useful for utilizing waste and hair of livestock (e.g. pig and cattle) to prepare peptone. The peptone is used in fermentation of butyric acid bacteria (claimed).    ADVANTAGE - The method cost-effectively utilizes waste resources.    DETAILED DESCRIPTION - Method of utilizing waste and hair of livestock (e.g. pig and cattle) to prepare peptone and application of peptone in fermentation of butyric acid bacteria, are claimed. The method involves (a) adding the hair of livestock (e.g. pig and cattle) to different buckets, immersing the livestock hair in tap water at 40-50 degrees C for 15-30 minutes, washing the livestock hair, and repeating above-mentioned step for 2-3 times, where the weight ratio of the livestock hair and tap water is 1:2-4, (b) blast drying the livestock hair at 50-60 degrees C for 4-8 hours, and respectively crushing 20-30 wt.% pig hair and 70-80 wt.% cattle hair using crusher to a particle size of 0.2-0.5 cm, (c) mixing 100 kg crushed pig hair and 300-500 kg crushed cattle hair with 8-15 % mixed acid solution, adding obtained mixture to furnace, cooking mixture using steam at 0.1-0.2 MPa and 150-180 degrees C for 3-6 hours, and carrying out acid hydrolysis, (d) cooling obtained hydrolyzate, maintaining the hydrolyzate at 45-55 degrees C, regulating pH of the hydrolyzate to 9-10 using sodium hydroxide, adding 0.1-0.3 wt.% enzyme powder, and carrying out enzymolysis for 1-3 hours, (e) regulating pH of enzymolyzed product to 6-7, heating enzymolyzed product at 75-85 degrees C for 5-10 minutes under stirring condition for inactivation of enzyme, and carrying out decolorization using activated coal for 1-1.5 hours to obtain product having 1-2% protein, (f) filtering the product using plate and frame filter press, collecting filtrate, and carrying out ultrafiltration using ultrafiltration membrane having pore size of 0.3-0.5 mu m for removing pathogens, (g) carrying out clarification using double-effect evaporator, and concentrating clarified product at 60-80 degrees C to obtain concentrated liquid, and (h) spray-drying the concentrated liquid by controlling air inlet temperature at 160-175 degrees C and air outlet temperature at 85-95 degrees C to obtain product. An INDEPENDENT CLAIM is included for peptone, which is prepared by above-mentioned method.