NOVELTY - An angola glycosides C, aucubin and harpagoside glycoside is prepared using high speed counter-current chromatography separation and purification by: crushing radix scrophulariae into rough powder, adding ethyl alcohol solvent, heating to reflux extract, hot filtering, cooling, and removing solvent under reduced pressure; and filling into macroporous resin chromatographic column, first eluting with water and ethyl alcohol mixed solvent, collecting, vacuum recovering ethyl alcohol, and drying to obtain angola glycosides C, aucubin and harpagide mixed crude extracts.    USE - Method for preparing angola glycosides C, aucubin and harpagoside glycoside using high speed counter-current chromatography separation and purification (claimed).    ADVANTAGE - The method avoids sample loss, has good separating effect, less solvent dosage and no pollution, and is efficient and rapid.    DETAILED DESCRIPTION - An angola glycosides C, aucubin and harpagoside glycoside is prepared using high speed counter-current chromatography separation and purification by:    (A) crushing radix scrophulariae, adding 30 vol.% ethyl alcohol solvent, heating to reflux extract for 1-3 times, hot filtering, cooling, and removing solvent under reduced pressure to obtain ethyl alcohol extract;    (B) filling into macroporous resin chromatographic column, first eluting with water and ethyl alcohol mixed solvent, collecting angola glycosides C, aucubin and harpagide, vacuum recovering ethyl alcohol, and drying to obtain angola glycosides C, aucubin and harpagide mixed crude extracts;    (C) high speed counter-current chromatography separating angola glycosides C crude extract with n-butanol, ethyl acetate and water at volume ratio of 4:6:10, standing, taking upper phase as fixed phase and lower phase as mobile phase, filling fixed phase with multilayer coil separation column of high speed counter-current chromatograph at rotating speed of 500-1000 revolutions/minute (rpm), injecting mobile phase at flow speed of 0.5-5 ml/minute, UV detecting at wave length of 190-380 nm, dissolving into mixed solvent at volume ratio of 1:1, collecting eluent containing angola glycosides C, concentrating, and drying to obtain angola glycosides C crude product;    (D) isocratic eluting with water and methyl alcohol mixed solvent at volume ratio of 1:1 at flow speed of 35 mL/minute, UV detecting at wavelength of 210 nm, collecting, concentrating under reduced pressure, and freeze-drying to obtain pure angola glycosides C;    (E) high speed counter-current chromatography separating aucubin and harpagide mixed crude extracts with n-butyl alcohol, ethyl alcohol, ammonium sulfate saturated solution and water at volume ratio of 2:7:8:8, standing, taking lower phase as fixed phase and upper phase as mobile phase, filling fixed phase with multilayer coil of high speed counter-current chromatography separation column, setting at rotating speed of 500-1000 rpm, injecting mobile phase at flow speed of 0.5-5 ml/minute, UV detecting at wavelength of 190-380 nm, dissolving into mixed solvent at volume ratio of 1:1, collecting effluent, detecting using high-performance liquid chromatography, combining effluent liquid, concentrating, and drying to obtain aucubin and harpagide crude products;    (F) chromatographic separating aucubin crude product at low pressure, gradient eluting with water and methanol mixed solvent at flow speed of 35 mL/minute, UV detecting at wavelength of 210 nm, detecting using high-performance liquid chromatography, combining, collecting, concentrating, and drying to obtain aucubin crude product B;    (G) purifying through liquid phase chromatography, eluting with methyl alcohol and water mixed solvent at volume ratio of 5:95 at flow speed of 3 mL/minute, UV detecting at wavelength of 210 nm, collecting, concentrating, and drying to obtain pure aucubin; and    (H) taking harpagide crude product, purifying through liquid phase chromatography, eluting with methyl alcohol and water at volume ratio of 12:88 at flow speed of 3 mL/minute, UV detecting at wavelength of 210 nm, concentrating, and drying to obtain pure harpagoside glycoside.