NOVELTY - Loop mediated isothermal gene amplification detection kit comprises reaction solution comprising 1:10 mmol/l deoxynucleotide triphosphates, 10X reaction buffer, 150 mmol/l magnesium sulfate, 5 mol/l betaine and sterilized double distilled water, reaction mixture comprising 2:10 mu mol outer primer 1 comprising base pair sequence, 1:10 mu mol outer primer 2 comprising base pair sequence, 2:40 mu mol inner primer 1 comprising base pair sequence, and 1:40 mu mol inner primer 2 comprising base pair sequence, 8 U/ mu l Bst DNA polymerase, 10% SYBR green I fluorescent dye.    USE - The loop mediated isothermal gene amplification kit is useful for detecting type B Clostridium botulinum (claimed).    ADVANTAGE - The loop mediated isothermal gene amplification kit enables to detect product within short period of time and without requirement of device, in a simple, convenient and environmentally-friendly manner.    DETAILED DESCRIPTION - Loop mediated isothermal gene amplification detection kit comprises reaction solution comprising 1:10 mmol/l deoxynucleotide triphosphates, 10X reaction buffer, 150 mmol/l magnesium sulfate, 5 mol/l betaine and sterilized double distilled water, reaction mixture comprising 2:10 mu mol outer primer 1 comprising base pair sequence of: gtgttccactcgaagagtt, 1:10 mu mol outer primer 2 comprising base pair sequence of: catattctgggcaaaacttca, 2:40 mu mol inner primer 1 comprising base pair sequence of: ttcgctccacttctcctggacaaacattgctagtgtaactgtt, and 1:40 mu mol inner primer 2 comprising base pair sequence of: acctgggccagttttaaatgaaaattatacccccgaagccttc, 8 U/ mu l Bst DNA polymerase, 10% SYBR green I fluorescent dye, where the loop mediated isothermal gene amplification kit comprises 19 mu l reaction solution comprising 10 mmol/l deoxynucleotide triphosphates, 10X reaction buffer, 150 mmol/l magnesium sulfate (1 mu l), 5 mol/l betaine (1 mu l) and sterilized double distilled water, and 3 mu l reaction mixture comprising outer primers 1 and 2 and inner primers 1 and 2. An INDEPENDENT CLAIM is included for loop mediated isothermal gene amplification detection method, which involves extracting to-be-detected DNA sample, and performing loop mediated isothermal gene amplification using DNA sample and reaction solution for 50-70 minutes, analyzing amplified product, if the product is green, then the result is positive and if the product is orange, then the result is negative.