NOVELTY - Cell immobilization of D-amino acid-producing bacteria is performed by inoculating bacteria stored at - 80 degrees C in 30 degrees C lysogeny broth (LB) liquid culture medium, culturing at 150 rpm for 24 hours to obtain activated strain, preparing NADF liquid culture medium, transferring activated strain to 20 ml seed NADF culture medium, shaking culturing at 28-30 degrees C and 150 rpm for 24 hours, inoculating in 50 ml NADF culture medium with inoculum amount of 10 vol.%, shaking culturing at 28-30 degrees C and 150 rpm for 48 hours, and centrifuging fermentation liquid at 4 degrees C and 5000 rpm for 10 minutes.    USE - Cell immobilization of D-amino acid-producing bacteria for preparing D-phenylalanine (claimed).    ADVANTAGE - Cell immobilization of D-amino acid-producing bacteria has low energy consumption, mild reaction condition, and small pollution.    DETAILED DESCRIPTION - Cell immobilization of D-amino acid-producing bacteria is performed by inoculating bacteria stored at - 80 degrees C in 30 degrees C lysogeny broth (LB) liquid culture medium, culturing at 150 rpm for 24 hours to obtain activated strain, preparing NADF liquid culture medium, transferring activated strain to 20 ml seed NADF culture medium, shaking culturing at 28-30 degrees C and 150 rpm for 24 hours, inoculating in 50 ml NADF culture medium with inoculum amount of 10 vol.%, shaking culturing at 28-30 degrees C and 150 rpm for 48 hours, centrifuging fermentation liquid at 4 degrees C and 5000 rpm for 10 minutes, discarding supernatant, washing 3 times with deionized water, centrifuging supernatant to reduce culture medium impurities, collecting bacteria cells, adding 0.5 g sodium alginate to 1 ml water, heating and stirring, preparing 4% calcium chloride (CaCl2) barium chloride (BaCl2) solution, sterilizing at 121 degrees C for 20 minutes, cooling to room temperature, mixing wet bacteria cells with sodium alginate, adding 4% CaCl2+BaCl2 solution to form immobilized particles with diameter of 1-3 mm, and storing at 4 degrees C in which NADF liquid culture medium contains 0.01 vol.% N-acyl-DL-phenylalanine, 0.5 vol.% yeast extract, 0.5 vol.% sodium chloride (NaCl), 0.1 vol.% monopotassium phosphate (KH2PO4), and 0.0005 vol.% magnesium sulfate heptahydrate (MgSO4.7H2O).    DESCRIPTION OF DRAWING(S) - The drawing shows a flowchart for the cell immobilization of D-amino acid-producing bacteria. (Drawing includes non-English language text).