NOVELTY - Cultivation of microbial agent for waste water purification treatment, involves: mixing Bacilli, sawdust and diatomite in weight ratio of 1:1:1, stirring uniformly, then below 4 degrees C low temperature drying, drying till moisture control in 10%, where the Bacilli mix contains: Bacillus subtilis, Paracoccus denitrificans, Alcaligenes faecalis, Acidithiobacillus ferrooxidans, Bacillus megaterium spore, copper green Pseudomonas, Bacillus cereus, Sphingomonas and Halomonas.    USE - In cultivation of microbial agent for waste water purification treatment (claimed).    ADVANTAGE - The microbial agent preparation has reasonably symbiotic coordination between each strain without antagonist. The preparation method is simple and easy. The operation method is simple and suitable for industrial production.    DETAILED DESCRIPTION - Cultivation of microbial agent for waste water purification treatment, involves: mixing Bacilli, sawdust and diatomite according to weight ratio of 1:1:1, stirring uniformly, then below 4 degrees C low temperature drying, drying till moisture control in 10%, where the Bacilli mix contains following raw materials: 8 parts by volume Bacillus subtilis, 8 parts by volume Paracoccus denitrificans, 6 parts by volume Alcaligenes faecalis, 6 parts by volume Acidithiobacillus ferrooxidans, 3 parts by volume Bacillus megaterium spore, 2 parts by volume copper green Pseudomonas, 2 parts by volume Bacillus cereus, 1 parts by volume Sphingomonas and 1 parts by volume Halomonas. The bacteria Bacilli concentration of the raw material are controlled 2x 108/ml. The sawdust or diatomite particle diameter is controlled at greater than or equal to 100 mesh. An INDEPENDENT CLAIM is included for preparation of fertilizer, involving: removing aquaculture wastewater solids through solid-liquid separation, and then adjusting pH of the wastewater to 7-8; according to per cubic meter of liquid each time adding the microbial agent prepared by the method, each day feeding 1 times, continuously adding three days, five days, standing the liquid and filtering through plate and frame filter, at last, discharging, at the same time, collecting the plate and frame filtrate and obtaining microorganism fermentation product, the compound 1, the corn straw pole drying, then crushing them into corn straw pole powder with a crusher, to add in the fermentation tank, then adding humic acid, grass carbon, zeolite powder and water, 1000 rotation/minute of speed stirring or 15 minutes, standing for 7 days to obtain the organic matter, mixing the organic matter and compound 1 according to 10:1 uniformly, low temperature drying; 10 degrees C granulating; where weight ratio of corn straw pole powder, humic acid, grass carbon, zeolite powder and water is 5:2:2:1: 0, and the grain diameter of corn straw pole powder, humic acid, grass carbon and zeolite powder are a more than 200 meshes.