NOVELTY - Micro-ecological fertilizer comprises the 1-5 pts. wt.Bacillus megaterium microbial inoculum, 16-25 pts. wt.Bacillus subtilisculture, 10-18 pts. wt. Aspergillus niger culture, 5-12 pts. wt.Lactobacillus agent. The Bacillus subtilis preservation number is (CGMCC No: 7926) and Aspergillus niger is (CGMCC No: 7927).    USE - Used as micro-ecological fertilizer.    ADVANTAGE - The fertilizer can enhance soil physical property and increase soil fertility.    DETAILED DESCRIPTION - INDEPENDENT CLAIM is also included for: (i)preparing Bacillus subtilisculture comprising (i) providing Bacillus subtilisslope strain access in 500 ml shake flask, adding 100 ml of culturing liquid, placing in an rotary shaking table at 180 rotations/minute, and culturing at 30 degrees C for 24 hours, (ii) inoculating 10% of cultured first stage seed in to the 500 ml shake flask, and culturing the seed,(iii) inoculating 10% of cultured second stage seed in to an 5000 ml shake flask, adding 1000 ml of culturing liquid, placing in an rotary shaking table at 100 rotations/minute, and culturing at 30 degrees C for 24 hours, (iv) inoculating 10% of cultured third level seed 150 L of seed tank, providing 100 l of fermentation medium, culturing at 28 degrees C, stirring at the speed of 100 rotations/minute, ventilating amount vol./vol. is 1:0.5, maintaining the pressure at 0.05 MPa, and culturing for 24 hours,(v) inoculating 1.5 ton total volume of 10% bacterial inoculums into the seed tank, adding 1 ton of fermentation inoculum, culturing at 28 degrees C, stirring at the speed of 100 rotations/minute, ventilating amount vol./vol. is 1:0.5, and maintaining pressure at 0.05 MPa, and culturing for 24 hours, where in culture medium composed of 6% glucose, 1% yeast extract, 0.2% peptone, 1% calcium carbonate, and the pH is 6.8; (ii)#preparing Aspergillus nigerculture comprising inoculating the spore in solid state fermentation culture, covering cultured fungus with culture medium at 26-33 degrees C, drying under low temperature fluidization bed, crushing the dried object to obtain solid state fermentation culture; and #preparing micro-ecological fertilizer where in preparing Lactobacillusagent comprising convention fermentation culture method obtaining seed liquid, Inoculating 3 ton of 5% bacterial strain in seed tank, adding 2 ton of fermentation culture medium, culturing at 28 degrees C for 22 hours, and maintaining the pressure at 0.05 MPa, concentrating 45% of fermentation broth at low temperature vacuum after fermentation, maintaining the pH at 6.8 and concentrating liquid to obtain fungus, adding carrier to concentrated liquid, mixing uniformly, and drying under fluidization bed at 50 degrees C, where in concentrated liquid and carrier weight ratio is 0.5:1, where in carrier comprises 25 pts. wt. calcium carbonate, 12 pts. wt. dextrin, where in culture medium comprises 1% peptone, 1% beef extract, 0.5% yeast extract, 0.5% glucose, 0.5% sodium acetate, 0.2% citric acid,1% Tween 800 (RTM: Puerarin), 0.2% dipotassium hydrogen phosphate, 0.02% magnesium sulfate heptahydrate, 0.005% manganese sulfate monohydrate, 2% calcium carbonate, and 1.5% agar.