NOVELTY - Acidithiobacillus ferrooxidans (CGMCC NO.7836) is new. Bioleaching high fluorine containing uranium comprising crushing the high fluorin uranium mine to less than 40 mm and carrying out the granularity, and stacking at high at 3-6 m.    USE - The strain is used for biological leaching.    ADVANTAGE - The method: increases the leaching rate of uranium; is economical; and is environmentally friendly.    DETAILED DESCRIPTION - INDEPENDENT CLAIM is also included for:    (1) the bacterial culture medium comprises 20.01-0.10 g/l calcium nitrate,0.50-20 g/l magnesium sulfate heptahydrate, 0.10-1 g/l potassium hydrogen phosphate trihydrate, 10-50 g/l aluminum sulfate octadecahydrate, 30-1.90 g/l iron sulfate, 60-10 g/l ammonium fluoride and 14.70-44.20 g/l iron sulfate heptahydrate; and    (2) bioleaching high fluorine containing uranium comprising (i) crushing the high fluorin uranium mine to less than 40 mm and carrying out the granularity, and stacking at high at 3-6 m,(ii) spraying using diluted sulfur acid and the pH is 1-2, dissolving fluorin ore, where in spraying amount is 0.05-0.20 m3/(t ore. time), and spraying for 12 hours, resin adsorbs leaching liquid with uranium after regulating the pH, carrying out for 5-25 days, pre-processing the acidification reaction for 5 days front and the pH value of spray leaching is less than 0.1, (iii) carrying out adsorption of the step (ii) obtained uranium using leaching liquid, (iv) regulating the pH value of step (iii) obtained acidified uranium using sulfuric acid and the pH value is 1.8-2.5, adding easily hydrolysable trivalent aluminum and the concentration of aluminum salt in solution is 0.0-12.0 g/l, mass concentration should be not less than 0.25 times of F-concentration, and mass concentration is more than trivalent aluminum and fluorin ion for complexing, and occupy fluorin, reducing fluorin and complex of trivalent iron complexes and reduced fluoride on inhibition of bacterial leaching, (v) using step (iv) regulating pH value of obtained solution to 1.8-2.5, adding 4.5-9 g/l ferrous sulfate, and preparing into culture medium, inoculating fluorin leaching ore strain in prepared culture medium, and inoculating amount is 10-30 %, leaching at 25-35 degrees C, and the inflation amount is 0.3-0.6 m3/m3 solution. minute, and oxidizing the solution, to obtain finished culture, (vi) spraying the ore stack to the step (iv) obtained leaching ore fluorin fungus nutrient solution and step (vi) extracting the uranium liquid obtained, where in spraying amount is 0.05-0.20 m3/t ore, and spraying for 12 hour, reducing ore plate, check the ore stack product position U is less than 0.02 wt.%,(vii) adsorbing the biological leaching liquid on resin containing uranium, extracting uranium tailing liquid and processing as culture medium.