NOVELTY - Preparing 7-aminocephalosporanic acid (ACA) immobilized enzyme LK118 involves polymerizing crosslinking monomer in suspension to obtain macroporous epoxy-type resin. The concentration of epoxy in macroporous epoxy-type resin is 300 mu mol/g, and has turbidity i.e. less than 200 and pore size i.e. greater than 50nm. The obtained macroporous epoxy-type resin is treated with phosphate buffer solution. The phosphate buffer solution is dissolved with cephalosporin-C acylase (CPC acylase). The obtained mixture is then added with macroporous epoxy-type resin at 20-30 degrees C with shaking for 15-40 hours.    USE - Method for preparing 7-ACA immobilized enzyme LK118 (claimed).    ADVANTAGE - The method enables to prepare durable 7-ACA immobilized enzyme LK118 that has high conversion rate and improved shelf life.    DETAILED DESCRIPTION - Preparing 7-aminocephalosporanic acid (ACA) immobilized enzyme LK118 involves polymerizing crosslinking monomer in suspension to obtain macroporous epoxy-type resin. The concentration of epoxy in macroporous epoxy-type resin is 300 mu mol/g, and has turbidity i.e. less than 200 and pore size i.e. greater than 50nm. The obtained macroporous epoxy-type resin is treated with phosphate buffer solution. The phosphate buffer solution is dissolved with cephalosporin-C acylase (CPC acylase). The obtained mixture is then added with macroporous epoxy-type resin at 20-30 degrees C with shaking for 15-40 hours. The obtained mixture is filtered to obtain precipitate, which is washed with phosphate buffer solution to obtain immobilized CPC acylase i.e. 7-ACA immobilized enzyme LK118. An INDEPENDENT CLAIM is included for a method for extracting 7-ACA, which involves:    (A) diluting CPC substrate concentrate to 25000-40000 units/mL titer value;    (B) adding 7-ACA immobilized enzyme LK118 (2-10 wt.%) to the obtained CPC concentrate;    (C) adding ammonia to adjust pH to 8.2-8.4;    (D) incubating the obtained mixture at 10-15 degrees C for 40-100 minutes to obtain 0-200 units/ml titer value;    (E) isolating the obtained product;    (F) cooling the obtained product to 0-10 degrees C;    (G) adjusting pH of the obtained product to 5-5.6 by adding 5-6mol/L hydrochloric acid solution; and    (H) washing and drying the obtained product to obtain 7-ACA.