NOVELTY - Culture medium comprises seed culture medium, and fermentation medium. The seed culture medium comprises maltose, starch, rapeseed oil, soybean meal, corn residue, corn protein powder, calcium carbonate, ammonium sulfate, and isomaltase. The fermentation culture medium comprises maltose, starch, rapeseed oil, soybean meal, corn residue, corn protein powder, potassium dihydrogen phosphate, calcium carbonate, ammonium sulfate, cyclohexyl formate, magnesium sulfate, zinc sulfate, polyethylene glycol, polystyrene resin, and isomaltase.    USE - The culture medium is useful for fermentative production of doramectin using Streptomyces avermitilis (all claimed).    ADVANTAGE - The medium is cost-effective and environmentally-friendly, and has high stability.    DETAILED DESCRIPTION - Culture medium comprises seed culture medium, and fermentation medium. The seed culture medium comprises 10-20 ml/l maltose, 30-40 g/l starch mixture, 5-10 ml/l rapeseed oil, 20-30 g/l soybean meal, 15-25 g/l corn residue, 15-20 g/l corn protein powder, 1-5 g/l calcium carbonate, 1-5 g/l ammonium sulfate, and 0.01-0.05 g/l isomaltase. The fermentation culture medium comprises 30-40 ml/l maltose, 30-50 g/l starch mixture, 10-15 ml/l rapeseed oil, 30-40 g/l soybean meal, 20-30 g/l corn residue, 20-30 g/l corn protein powder, 0.8-1.2 g/l potassium dihydrogen phosphate, 1.5-8 g/l calcium carbonate, 3-7 g/l ammonium sulfate, 0.5-1.5 g/l cyclohexyl formate, 0.2-0.6 g/l magnesium sulfate, 0.3-0.7 g/l zinc sulfate, 0.04-0.08 ml/l polyethylene glycol, 2-6 g/l polystyrene resin, and 0.05-0.09 g/l isomaltase. An INDEPENDENT CLAIM is included for method for fermentative production of doramectin, which involves inoculating strain to seed culture medium, allowing the strains to grow for 24 hours to obtain seed culture, culturing the seed culture in fermentation culture medium for 30-80 hours, controlling pH value of culture at less than 6.5, adding sodium hydroxide solution to increase pH to 6.5-7, and recovering doramectin from the culture.