NOVELTY - Kit and method for detecting human epidermal growth factor receptor (EGFR) gene mutation, are claimed. The kit comprises peptide nucleic acid (PNA)/locked nucleic acid (LNA) probe as blocking probe, fluorescent hybridization probe for determining melting curve result, 1x PCR reactants, tris(hydroxymethyl)aminomethane-chloride (cl), potassium chloride (Kcl), magnesium chloride (MgCl2), hot start polymerase, uracil n-glycosylase (UNG) enzyme, and lyoprotectant composition.    USE - The method and kit are useful for detecting human EGFR gene mutation (claimed).    ADVANTAGE - The method is simple, environmentally-friendly and convenient to perform and transport.    DETAILED DESCRIPTION - Method and kit for detecting human epidermal growth factor receptor (EGFR) gene mutation, are claimed. The kit comprises peptide nucleic acid (PNA)/locked nucleic acid (LNA) probe as blocking probe, fluorescent hybridization probe for determining melting curve result, 1x PCR reactants, tris(hydroxymethyl)aminomethane-chloride (cl), potassium chloride (Kcl), magnesium chloride (MgCl2), hot start polymerase, uracil n-glycosylase (UNG) enzyme, and lyoprotectant composition. The method involves (a) designing special primers to perform multi-single-stranded PCR amplification, where the primers are chosen from SEQ ID NOs: 1-8, (b) designing probes which have excellent specificity with respect to changes in value of the melting curve to determine mutation, where the probes are chosen from SEQ ID NOs: 17-20 (sequence not defined in the specification), (c) storing the PCR reagent in solid form, (d) optimizing reaction conditions which is divided into two phases, where the first phase is enriched phase using primers in very low concentrations and excess of forward primer, where the PCR reaction is performed at 50 degrees C to produce large number of single-stranded nucleotide sequence of target, and (e) blocking expression in exon 19 (wild-type expression) using PNA blocking probe. catggtgagggctg(a/g/t/a)(a/g/t/a)(a/g/t/a)(a/g/t/a)(a/g/t/a)aggtga (SEQ ID NO: 1), ccccaccagaccat(a/g/t/a)(a/g/t/a)(a/g/t/a)(a/g/t/a)(a/g/t/a)gagagg (SEQ ID NO: 2), gtgcatcgctggtaa(a/g/t/a)(a/g/t/a)(a/g/t/a)(a/g/t/a)(a/g/t/a)catcca (SEQ ID NO: 3), ggagatgagcagggtc(a/g/t/a)(a/g/t/a)(a/g/t/a)(a/g/t/a)(a/g/t/a)tagagca (SEQ ID NO: 4), cgcattcatgcgtct(a/g/t/a)(a/g/t/a)(a/g/t/a)(a/g/t/a)(a/g/t/a)tcacc (SEQ ID NO: 5), ctatcccaggagcg(a/g/t/a)(a/g/t/a)(a/g/t/a)(a/g/t/a)(a/g/t/a)cagacc (SEQ ID NO: 6), tggcatgaacatga(a/g/t/a)(a/g/t/a)(a/g/t/a)(a/g/t/a)(a/g/t/a)ccctgaa (SEQ ID NO: 7) and cagcctggtccct(a/g/t/a)(a/g/t/a)(a/g/t/a)(a/g/t/a)(a/g/t/a)ggtgtc (SEQ ID NO: 8).