NOVELTY - Separation and purification of jack pine elements and caffeic acid derivatives from Chinese propolis aqueous extract includes freezing Chinese propolis, crushing, heat extracting with distilled water, filtering, obtaining water extract, naturally cooling to room temperature, decompression concentrating the lower layer water solution, adding the concentrated liquid with ethyl alcohol, standing for one night, taking supernatant to obtain concentrated extract, adding water, dissolving, adding in macroporous resin column, eluting with water and gradient eluting with ethanol-water solution.    USE - The method is for separation and purification of jack pine elements and caffeic acid derivatives from Chinese propolis aqueous extract. It is used for separating 3,4-dimethoxy cinnamic acid, jack pine elements, caffeic acid benzyl ester and caffeic acid phenethyl ester. (all claimed)    ADVANTAGE - The method provides high purity components, is green and environment-friendly, provides no serious environmental damage and has low total cost.    DETAILED DESCRIPTION - Separation and purification of jack pine elements and caffeic acid derivatives from Chinese propolis aqueous extract comprises taking Chinese propolis, freezing, crushing, heat extracting with distilled water, filtering, obtaining water extract, naturally cooling to room temperature, decompression concentrating the lower layer water solution to a relative density of 1.2, adding the concentrated liquid with 95% ethyl alcohol until alcohol content is 70%, standing for one night, taking supernatant to obtain concentrated extract, adding water, dissolving, adding in macroporous resin column, eluting with water, gradient eluting with 20%, 40%, 60%, 80% and 95% ethanol-water solution as mobile phase, respectively, collecting 40% and 60% ethanol eluate, concentrating to obtain sample 1 and 2, subjecting sample 1 to semi-preparative HPLC using 18C simulated moving bed (SMB) 100 chromatographic column, where mobile phase is methanol-water (50:50 vol/vol.) and detection wavelength is 280 nm, collecting target component fraction, decompression concentrating, separating monomer compound (3,4-dimethoxy cinnamic acid and jack pine elements), subjecting sample 2 to semi-preparative HPLC for separation purification using 18C SMB 100 chromatographic column, where mobile phase is methanol-water (60:40 vol/vol.) and detection wavelength is 280 nm, collecting target component fraction, decompression concentrating and separating monomer compound (jack pine element, caffeic acid benzyl ester and caffeic acid phenethyl ester).