NOVELTY - Preparing Acanthopanax senticosus extract comprises (i) extracting Acanthopanax senticosus medicinal material with water and concentrating; (ii) carrying out primary alcohol precipitation and concentration to obtain primary alcohol precipitation paste; (iii) adjusting the pH with lime milk and sulfuric acid, allowing to stand and concentrating; (iv) performing secondary alcohol precipitation and concentration; and (v) diluting, carrying out macroporous adsorbent resin adsorption and elution, subjecting the eluent to secondary ultrafiltration, and concentrating.    USE - The method is useful for preparing Acanthopanax senticosus extract (claimed).    ADVANTAGE - The method reduces the content of nonpolar impurities, improves the purity of effective components in the extract, reduces the energy consumption and improves extract yield. The extract is safe and stable.    DETAILED DESCRIPTION - Preparing Acanthopanax senticosus extract comprises (i) extracting Acanthopanax senticosus medicinal material with water and concentrating the extracted solution to obtain aqueous decoction paste; (ii) carrying out primary alcohol precipitation and concentration to obtain primary alcohol precipitation paste; (iii) adjusting the pH with lime milk and sulfuric acid, allowing to stand and concentrating to obtain a concentrated paste; (iv) performing secondary alcohol precipitation and concentration to obtain secondary alcohol precipitation paste; and (v) diluting the secondary alcohol precipitation paste, carrying out macroporous adsorbent resin adsorption and elution, subjecting the eluent to secondary ultrafiltration, and concentrating to obtain the Acanthopanax senticosus extract.    INDEPENDENT CLAIMS are also included for:    (1) Acanthopanax senticosus extract prepared by the above method, where the total flavonoid content is 50-65 mg/g, preferably 53-63 mg/g, syringin content is 5.5-7.5 mg/g, preferably 5.7-6.7 mg/g and eleutheroside E content is 4.5-6.0 mg/g, preferably 4.5-5.2 mg/g, and    (2) preparation, comprising the Acanthopanax senticosus extract and a carrier or a diluent.