NOVELTY - Isolated or purified polypeptide comprising endoglucanase activity improved with respect to the endoglucanase activity of the reference protein endoglucanase 2, is new. The polypeptide is e.g. amino acid sequence including SEQ ID NOs: 4, 8 10, 12, 14, 16, 18, 20, 22, 30 or 32 (each comprising a fully defined sequence of 418 amino acids as given in the specification), or SEQ ID NO: 6, 24, 26 or 34 (each comprising a fully defined sequence of 419 amino acids as given in the specification), or SEQ ID NO: 28 (comprising a fully defined sequence of 415 amino acids as given in the specification).    USE - The isolated or purified polypeptide is useful in an enzyme composition for hydrolyzing cellulose for producing biofuel (all claimed).    ADVANTAGE - The isolated or purified polypeptide provides biofuel in a cost effective manner, by decreasing the cost of enzymatic hydrolysis.    DETAILED DESCRIPTION - Isolated or purified polypeptide comprising endoglucanase activity improved with respect to the endoglucanase activity of the reference protein endoglucanase 2, is new. The polypeptide is: an amino acid sequence including SEQ ID NOs: 4, 8 10, 12, 14, 16, 18, 20, 22, 30 or 32 (each comprising a fully defined sequence of 418 amino acids as given in the specification), or SEQ ID NO: 6, 24, 26 or 34 (each comprising a fully defined sequence of 419 amino acids as given in the specification), or SEQ ID NO: 28 (comprising a fully defined sequence of 415 amino acids as given in the specification); and an amino acid sequence having a percentage identity of at least 70%, preferably at least 99%, relative to the sequence of SEQ ID NOs: 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32 or 34. INDEPENDENT CLAIMS are also included for:    (1) a purified or isolated nucleic acid encoding at least one isolated or purified polypeptide;    (2) a vector comprising the nucleic acid;    (3) an isolated host cell comprising the nucleic acid or the vector;    (4) enzyme composition capable of acting on the lignocellulosic biomass comprising at least one polypeptide; and    (5) producing biofuel from biomass, comprising either (a) suspending biomass in the aqueous phase to hydrolyze, (b) carrying out hydrolysis in the presence of the enzyme composition of the lignocellulosic biomass so as to produce a hydrolyzate containing glucose, (c) glucose fermentation of the hydrolyzate to produce a fermentation broth, and (d) separating biofuel from the fermentation broth, or step (a) as above per se, simultaneously adding the enzyme composition and a fermenting organism to produce a fermentation broth, and step (d) as above per se, or step (a) as above per se, adding the host cell with the fermenting organism and/or the enzyme composition to produce a fermentation broth, and step (d) as above per se.