NOVELTY - Neutrophil gelatinase-associated lipocalin (NGAL) rapid detection reagent comprises substrate, lid cover, and substrate sheet. The substrate is orderly processed with sample injector area, reaction area, detection area and waste liquid area which comprises micro flow channels. The sample injector area is provided with polyester film for filtering biological sample. The reaction area is coated with dried fluorescent microsphere labeled antibody containing fluorescent microsphere labeled NGAL polyclonal antibody and fluorescent microsphere labeled goat anti-mouse immunoglobulin G (IgG) antibody.    USE - Neutrophil gelatinase-associated lipocalin rapid detection reagent.    ADVANTAGE - The detection reagent has simple and easy operation, fast detection, high sensitivity, good accuracy, and low cost. Its production process is environment friendly.    DETAILED DESCRIPTION - NGAL rapid detection reagent comprises substrate, lid cover, and substrate sheet. The substrate is orderly processed with sample injector area, reaction area, detection area and waste liquid area which comprises micro flow channels. The sample injector area is provided with polyester film for filtering biological sample. The reaction area is coated with dried fluorescent microsphere labeled antibody containing fluorescent microsphere labeled NGAL polyclonal antibody and fluorescent microsphere labeled goat anti-mouse IgG antibody. The detection area is provided with detection line containing NGAL polyclonal antibody and control line containing mouse IgG antibody. The waste liquid area includes capillary action. An INDEPENDENT CLAIM is included for preparation of NGAL rapid detection reagent comprising preparing fluorescent microsphere labeled NGAL polyclonal antibody and fluorescent microsphere labeled goat anti-mouse IgG antibody; preparing substrate and cover sheet of micro flow channel; preparing sample injector area, reaction area, detection area and waste liquid area; and assembling reagent.