NOVELTY - A Myriophyllum propagating method involves selecting growth robust, bright green color and pest free stem segments as explants, soaking the explants in an alkaline detergent and rinsing in tap, and repeatedly washing with distilled water and placing in sterile environment. The treated explant is sterilized aseptically with ethanol and mercuric chloride and rinsed. Leaves in the Myriophyllum explants are removed and placed in an induction medium, a proliferation culture medium and rooting culture medium. The obtained explant is hardened and transplanted.    USE - Method for propagating Myriophyllum.    ADVANTAGE - The method enables propagating the Myriophyllum in a rapid and eco-friendly manner.    DETAILED DESCRIPTION - A Myriophyllum propagating method involves selecting growth robust, bright green color and pest free stem segments as explants, soaking the explants an alkaline detergent for 30 minutes and rinsing in tap for 2 hours, and repeatedly washing with distilled water for 4-5 times, repeatedly washing with sterile water for 4-5 times and placing in sterile environment. The treated explant is sterilized aseptically with 70% ethanol for 15 seconds and rinsed with sterile water for 4-5 times, and sterilized with 0.1% mercuric chloride for 2-5minutes and rinsed with sterile water for 4-5 times. Leaves in the 5-2 cm Myriophyllum explants are removed and placed in an induction medium for culturing callus and shoots, where the induction medium comprises MS solid culture base, 0.50-2 mg/l 6-benzylaminopurine (6-BA), 0.25-0.50 mg/l naphthaleneacetic acid (NAA) and 3% sucrose. The induced explant is placed in a proliferation culture medium comprising 1/2 MS broth, 0.10 mg/l NAA and 1.5% sucrose. The proliferated explant is placed in a rooting culture medium comprising 1/2 MS broth, 0.50-2 mg/l 6-BA, 0.25-0.50 mg/l NAA and 1.5% sucrose. The rooted explant is hardened at room temperature for 1-2 days for removing shoots. The culture of roots is washed and transplanted.