NOVELTY - Preparing Grifola frondosa cultivation material, comprises (i) drying wooden chips, soybean meal, mushroom stalk, lime and gypsum, and pulverizing; (ii) mixing mushroom slag leachate with wooden chips, mixing with soybean cake, stacking, covering with grass straw mat, and allowing to naturally ferment; (iii) turning material once a day, and adding lime and gypsum; and (iv) fermenting cultivation material, loading to polypropylene bags, packaging, inoculating fungal strains, placing in dark room, controlling temperature and humidity, culturing, and subjecting to fruiting management.    USE - The method is useful for preparing Grifola frondosa cultivation material (claimed).    ADVANTAGE - The method utilizes mushroom slag leachate as a natural nutrient solution, containing carbohydrates, amino acids, peptides, nucleic acids and trace elements, and varieties of biological hormones, and which promotes growth of beneficial microbes, improves feedstock of microflora, and increases the catalytic decomposition of cultivation material; produces fermented material exhibiting high activity of beneficial bacteria and its active metabolite, promotes rapid growth of mycelium, and having good anti-bacterial effect; saves manpower, coal and other resources; and is environmental friendly, and thus it meets the low carbon environmental protection requirements.    DETAILED DESCRIPTION - Preparing Grifola frondosa cultivation material, comprises (i) taking 80-88% wooden chips, 8-12% soybean meal, 3-4% mushroom stalk, 1.5% lime and 1% gypsum, accounting to quality of dry matter, where the wooden chips used is 3-5 mm diameter hardwood wooden chips, and the mushroom stalk used is shiitake mushroom waste, and then drying the raw materials, and pulverizing into particles of 2-3 mm; (ii) adding water to mushrooms stalk particles in an amount of 20-30 times at room temperature, and soaking for 18-24 hours, uniformly mixing mushroom slag leachate with wooden chips, mixing with soybean cake, stacking the materials to a height of 1-1.2 m, and width of 2-3 m, and allowing to stack upto a length of ladder, controlling the dry weight of each stack of culture medium at 300-500 kg, and terminating the stalking process, and tapping for 4 weeks, then plugging wooden stick vertically to a diameter of 10-15 cm into a number of vertical vent plugs, and stacking straight into the bottom, where the pore density is 1/m3, and finally covering with grass straw mat, and allowing to naturally ferment; (iii) maintaining the temperature for 24 hours, once the material temperature reaches 60 degrees C, and then turning the material once a day, adding lime while turning for first time, and adding gypsum while turning for second time, and turning for 3-4 times totally, and allowing to last for 5-6 days, terminating the fermentation process, and obtaining cultivation material having moisture content of 63-65%; and (iv) fermenting the cultivation material, cooling, loading into polypropylene bags of dimensions 17x 35x 0.004 cm, and marking the inoculation pore, packaging, inoculating fungal strains into second end of pores in the bag, then discharging the cultivation bags into a dark room, controlling temperature at 20-22 degrees C, controlling humidity at 60-75%, and culturing for 24-26 days, allowing the mycelium to cover the bag, and subjecting to fruiting management.