NOVELTY - A recombinant Sporidiobolus strain comprising at least one expression cassette functional in Sporidiobolus that is stably integrated into its genome for the production of homologous or heterologous proteins, is new.    USE - The recombinant Sporidiobolus strain is for use in the production of homologous or heterologous proteins; in producing a recombinant cell used in producing oil; for a method of combining traits from various strains into a single cell-line; and for a method of extracting recombinant cells suitable in production of biofuels from an aqueous fermentation broth (all claimed).    ADVANTAGE - The recombinant Sporidiobolus strain can be effectively used to select, manipulate and improve fermentation organisms for use in the production of biofuels. Also, it has high yield and productivity characteristics and subsequently improves and optimizes the selected fermentation organisms for use in the production of biofuels.    DETAILED DESCRIPTION - INDEPENDENT CLAIMS are included for: (1) a method for producing a recombinant Sporidiobolus strain which comprises: (a) generating protoplasts of Sporidiobolus; (b) transforming the protoplasts generated in step (a) with at least one vector comprising an expression cassette encoding a homologous or heterologous protein; (c) selecting recombinant Sporidiobolus transformed in step (b) under specific culture conditions; and (d) recovering recombinant Sporidiobolus containing the expression cassette of the vector stably integrated into their genome; (2) a recombinant cell derived from a protoplast of the species Sporidiobolus and genetically transformed with a nucleic acid sequence; (3) residual biomass from the recombinant cell; (4) oil produced from the recombinant cell; (5) an expression vector construct for the production of a polypeptide in Sporidiobolus comprising: (a) a nucleic acid sequence encoding the polypeptide; and (b) nucleic acid sequences allowing for expression of the polypeptide in Sporidiobolus; (6) a Sporidiobolus cell comprising the vector construct; (7) a Sporidiobolus host cell, where the host cell has been modified to reduce or eliminate expression of a functional gene product of a nucleic acid sequence encoding a polypeptide having the amino acid sequence set forth in SEQ ID NO: 72 and/or SEQ ID NO: 82, sequences not defined here may be found at ftp://ftp.wipo.int/pub/published_pct_sequences/publication; (8) a method for combining traits from various strains into a single cell-line, which comprises: (a) generating protoplasts of each strain of Sporidiobolus of interest; (b) forcing the protoplasts generated in step (a) to artificially fuse; and (c) harvesting surviving fused cells; and (9) a method of extracting recombinant cells suitable in production of biofuels from an aqueous fermentation broth, which comprises: extracting recombinant cells from the aqueous fermentation broth, where the broth contains oleaginous microorganisms, leaving residual biomass solids of recombinant cells that expressed one or more genes selected from malic enzyme, acetyl-CoA synthetase, ATP citrate lyase, and phytoene dehydrogenase genes after oil extraction, and residual broth water.