NOVELTY - Constructing binary complex microflora comprises taking Alcaligenes faecalis (CGMCC NO: 1.2098) (PR1) and Acinetobacter calcoaceticus (CGMCC NO: 1.6186) (PR2) as raw material strain, inoculating PR1 and PR2 nutrient broth agar slant into 100 ml beef extract peptone liquid culture medium, activating at 30 degrees C in shaker at 150 rotations/minute for 24 hours, inoculating activated PR1 and PR2 inoculum into phenol-containing acclimation culture medium, culturing at 30 degrees C in shaker at 150 rotations/minute to obtain acclimation strain, repeating process for second cycle acclimation.    USE - The binary complex microflora is useful for phenol biodegradation in wastewater (claimed).    DETAILED DESCRIPTION - Constructing binary complex microflora comprises either: (i) taking Alcaligenes faecalis (CGMCC NO: 1.2098) (PR1) and Acinetobacter calcoaceticus (CGMCC NO: 1.6186) (PR2) as raw material strain, (ii) inoculating PR1 and PR2 nutrient broth agar slant into 100 ml beef extract peptone liquid culture medium, activating at 30 degrees C in a shaker at 150 rotations/minute for 24 hours, (iii) inoculating 10 ml activated PR1 and PR2 inoculum into phenol-containing acclimation culture medium, culturing at 30 degrees C in a shaker at 150 rotations/minute to obtain acclimation strain, repeating the process for second cycle acclimation for 24 hours, carrying out totally 5 cycles for 120 hours, adding 1.5 ml filter sterilized 10% (wt./vol.) phenol solution during first two acclimation period, adding 3 ml filter sterilized 10% (wt./vol.) phenol solution during third acclimation period and adding 8 ml filter sterilized 10% (wt./vol.) phenol solution during last two acclimation period, (iv) culturing the acclimation strain using dilution method to obtain single colony on the plate, taking a single colony and inoculating into slant medium, culturing until the colony grows, then storing in 4 degrees C refrigerator, (v) inoculating the strain into beef extract peptone solid medium using streak plate method, culturing in a biochemical incubator at 30 degrees C for 24 hours, observing the colony morphology, determining the color, transparency, surface smoothness and edge shape of the colonies, then distinguishing the PR1 and PR2 strains of binary complex microflora, (vi) inoculating PR1 and PR2 into beef extract peptone liquid medium, culturing at 30 degrees C in a shaker at 150 rotations/minute for 24 hours, placing the culture in a desktop high-speed refrigerated centrifuge at 10000 rotations/minute for 5 minutes, discarding the supernatant, re-suspending using 0.1 mol/l disodium phosphate-monosodium phosphate buffer (pH 8), centrifuging, discarding the supernatant, washing the cells twice, re-suspending using 0.1 mol/l disodium phosphate-monosodium phosphate buffer (pH 8), determining the optical density to 1.2 at 600 nm to obtain bacterial suspension, then refrigerating, simultaneously determining the viable cell number of PR1 and PR2 in bacterial suspension respectively by plate count method, and (vii) measuring the PR1 and PR2 viable cell count, drawing PR1 and PR2 bacterial suspension, mixing the PR1 and PR2 (total viable bacterial suspension of 2x 108 cfu/ml), with viable PR1 and PR2 initial rates of 0/10, 1/9, 2/8, 3/7, 4/6, 5/5, 6/4, 7/3, 8/2, 9/1 and 10/0, where 10/0 system represents PR1 and 0/10 system represents PR2; (viii) inoculating 10% (vol./vol.) mixed bacterial suspension in 200 mg/l phenol-containing inorganic salt culture medium, culturing at 30 degrees C in a shaker at 150 rotations/minute for 12 hours, simultaneously determining morphological characteristics by doubling dilution method, measuring the number of PR1 and PR2 to obtain vaccination PR1 system; or (a) taking Pseudomonas putida (CGMCC NO: 1.1003) (BA1) and Acinetobacter calcoaceticus (CGMCC NO: 1.3615) (BA2) as raw material strain, (b) inoculating BA1 and BA2 nutrient broth agar slant into 100 ml beef extract peptone liquid culture medium, activating at 30 degrees C in a shaker at 150 rotations/minute for 24 hours, (c) inoculating 10 ml activated BA1 and BA2 inoculum into phenol-containing acclimation culture medium, culturing at 30 degrees C in a shaker at 150 rotations/minute to obtain acclimation strain, repeating the process for second cycle acclimation for 24 hours, carrying out totally 5 cycles for 120 hours, adding 1.5 ml filter sterilized 10% (wt./vol.) phenol solution during first two acclimation period, adding 3 ml filter sterilized 10% (wt./vol.) phenol solution during third acclimation period and adding 8 ml filter sterilized 10% (wt./vol.) phenol solution during last two acclimation period, (d) culturing the acclimation strain using dilution method to obtain single colony on the plate, taking a single colony and inoculating into slant medium, culturing until the colony grows, then storing in 4 degrees C refrigerator, (e) inoculating the strain into beef extract peptone solid medium using streak plate method, culturing in a biochemical incubator at 30 degrees C for 24 hours, observing the colony morphology, determining the color, transparency, surface smoothness and edge shape of the colonies, then distinguishing the BA1 and BA2 strains of binary complex microflora, (f) inoculating BA1 and BA2 into beef extract peptone liquid medium, culturing at 30 degrees C in a shaker at 150 rotations/minute for 24 hours, placing the culture in a desktop high-speed refrigerated centrifuge at 10000 rotations/minute for 5 minutes, discarding the supernatant, re-suspending using 0.1 mol/l disodium phosphate-monosodium phosphate buffer (pH 8), centrifuging, discarding the supernatant, washing the cells twice, re-suspending using 0.1 mol/l disodium phosphate-monosodium phosphate buffer (pH 8), determining the optical density to 1.2 at 600 nm to obtain bacterial suspension, then refrigerating, simultaneously determining the viable cell number of BA1 and BA2 in bacterial suspension respectively by plate count method, and (g) measuring the BA1 and BA2 viable cell count, drawing BA1 and BA2 bacterial suspension, mixing the BA1 and BA2 (total viable bacterial suspension of 2x 108 cfu/ml), with viable BA1 and BA2 initial rates of 0/10, 1/9, 2/8, 3/7, 4/6, 5/5, 6/4, 7/3, 8/2, 9/1 and 10/0, where 10/0 system represents BA1 and 0/10 system represents BA2; (viii) inoculating 10% (vol./vol.) mixed bacterial suspension in 200 mg/l phenol-containing inorganic salt culture medium, culturing at 30 degrees C in a shaker at 150 rotations/minute for 12 hours, simultaneously determining morphological characteristics by doubling dilution method, measuring the number of BA1 and BA2 to obtain vaccination BA1 system.