TECHNOLOGY FOCUS - POLYMERS - Preparation: No general preparation given. Preferred Composition: The sample comprises cells. The phosphonous acid-containing oxolane derivative is chosen from (2-(((6-((4-carboxy-3-(6-hydroxy-3-oxo-3H-xanthen-9-yl)phenyl)formamido)-2-((phosphonatooxy)methyl)hexyl) phosphonato)oxy)ethyl) (2-(4-((4-carboxy-3-(6-hydroxy-3-oxo-3H-xanthen-9-yl)phenyl)formamido)butyl)-3-hydroxypropyl) phosphate, 2-(((2-(((2-(((3-(((3-(((3-(phosphonatooxy)-2-(pyren-1-ylmethyl)propyl) phosphonato)oxy)-2-(pyren-1-ylmethyl)propyl) phosphonato)oxy)-2-(pyren-1-ylmethyl)propyl) phosphonato)oxy)ethyl) phosphonato)oxy)ethyl) phosphonato)oxy)ethan-1-ol, 16-(3-hydroxy-2-(((2-(((2-(phosphonatooxy)ethyl) phosphonato)oxy)ethyl) phosphonato)oxy)propyl)-16-azahexacyclo(12.6.2.12,6.011,21.018,22.010,23)tricosa-1(21),2(23),3,5,7,9,11,13,18(22),19-decaene-15,17-dione, 16-(3-(((2-(((2-hydroxyethyl) phosphonato)oxy)ethyl) phosphonato)oxido)-2-(((2-(((2-(phosphonatooxy)ethyl) phosphonato)oxy)ethyl) phosphonato)oxy)propyl)-16-azahexacyclo(12.6.2.12,6.011,21.018,22.010,23)tricosa-1(21),2(23),3,5,7,9,11,13,18(22),19-decaene-15,17-dione, 16-(3-hydroxy-2-(((2-(((2-(((2-(((2-(((2-(((2-(phosphonatooxy)ethyl) phosphonato)oxy)ethyl) phosphonato)oxy)ethyl) phosphonato)oxy)ethyl) phosphonato)oxy)ethyl) phosphonato)oxy)ethyl) phosphonato)oxy)propyl)-16-azahexacyclo(12.6.2.12,6.011,21.018,22.010,23)tricosa-1(21),2(23),3,5,7,9,11,13,18(22),19-decaene-15,17-dione, 16-(3-(((2-(((2-(((2-(2-aminoethoxy)ethyl) phosphonato)oxy)ethyl) phosphonato)oxy)ethyl) phosphonato)oxy)-2-(((2-(((2-(phosphonatooxy)ethyl) phosphonato)oxy)ethyl) phosphonato)oxy)propyl)-16-azahexacyclo(12.6.2.12,6.011,21.018,22.010,23)tricosa-1(21),2(23),3,5,7,9,11,13,18(22),19-decaene-15,17-dione, 3-(phosphonatooxy)-2-(pyren-4-ylmethyl)propan-1-ol, 8-(dimethylamino)-3-(3-hydroxy-2-((phosphonatooxy)methyl)propyl)-3-azatricyclo(7.3.1.05,13)trideca-1(13),5,7,9,11-pentaene-2,4-dione, 2-(perylen-3-ylmethyl)-3-(phosphonatooxy)propan-1-ol, 3-(((3-(phosphonatooxy)-2-(pyren-4-ylmethyl)propyl) phosphonato)oxy)-2-(pyren-4-ylmethyl)propan-1-ol, and 1-(5-ethyl-4-(((3-hydroxy-2-(pyren-1-ylmethyl)propoxy)phosphinato)oxy)oxolan-2-yl)-5-methyl-1,3-diazinane-2,4-dione derivatives of formulae (II)-(VIII), (X), (XII)-(XIV), (XVI), (XVIII) and (XX).a=2;b=3;c=4;d=5;A=group of formula (IX);B'=group of formula (XI);C'=group of formula (XV);D'=group of formula (XVII);E=group of formula (XIX);F'=group of formula (XXI);andG=group of formula (XXII).Preferred Process: The staining of sample further involves observing the cells by flow cytometry, and distinguishing the fluorescence response from that of fluorophore (f2) having detectably different optical properties. The visual detection of biomolecules involves admixing phosphonous acid-containing oxolane derivative with one or more biomolecules, and detecting the phosphonous acid-containing oxolane derivative by its visible properties. The optical response is fluorescent response. Preferred Definitions:    M1=group chosen from groups of formulae (i)-(v);and    M2=group chosen from groups of formulae (vi)-(xiii).    EXAMPLE - The dyes were synthesized by standard solid phase DNA method was as follows, total phosphoramidite monomers were dissolved in 0.1 M acetonitrile-dichloromethane solution, and added in order using synthesis cycles which involves removing 5'-dimethoxytrityl protecting group with dichloroacetic acid in toluene, coupling next phosphoramidite with activator reagent in acetonitrile, oxidizing with iodine/pyridine/water, and capping with acetic anhydride/1-methylimidizole/acetonitrile. The synthesis cycle was repeated until 5'-oligofloroside was assembled. At the end of chain assembly, monomethoxytrityl group or dimthoxytrityl group was removed with dichloroacetic acid in dichloromethane or dichloroacetic acid in toluene. The dyes were cleaved from the solid support using concentrated aqueous ammonium hydroxide at room temperature for 2-4 hours. The product was concentrated in vacuum, and Sephadex G-25 (RTM: crosslinked dextran gel) columns were used to isolate the main product which was analyzed by RP-HPLC. Sequences, of representative oligomers were prepared, and spectral properties and molecular weights were determined by electrospray mass spectrometry.