NOVELTY - Preparation of vegetable cultivation substrate using earthworms hydrolysate involves taking poultry livestock manure pile, stacking, inoculating earthworms and feces, covering with wheat bran, conducting vermiculture, and separating earthworm; taking inoculated earthworms, washing, breaking earthworm tissue, adjusting pH, adding subtilisin, and performing hydrolysis; pouring hydrolysis reaction mixture into magnetic stirrer, water bathing, and standing; taking supernatant, centrifuging, and taking supernatant as hydrolysate; and mixing hydrolysate and vermicompost, adding peat, and stirring.    USE - Method for preparing vegetable cultivation substrate using earthworms hydrolysate (claimed).    ADVANTAGE - The product contains large amount of amino acids and proteins, which improves vegetable cultivation survival rate at more than 88%.    DETAILED DESCRIPTION - Preparation of vegetable cultivation substrate using earthworms hydrolysate comprises taking 2-3 kg poultry livestock manure pile, stacking at 20-30 cm thick for 5-7 days, maintaining temperature at 25-35 degrees C, inoculating earthworms and feces at 1:200-1:300 mass ratio, covering with 2-3 cm thick wheat bran, maintaining relative humidity at 55-75%, controlling temperature at 25-30 degrees C, conducting vermiculture, and separating earthworm to obtain vermicompost as culture medium substrate; taking 100-150 g inoculated earthworms, washing with deionized water, spitting earthworm food residue, breaking earthworm tissue using high-speed broken machine, mixing earthworm and deionized water at 1:1-1:2 mass ratio, adjusting pH to 6-8 by adding 0.02 mol/L dilute hydrochloric acid, adding 300-500 mg subtilisin, and performing hydrolysis at 45-55 degrees C for 48-60 hours; pouring hydrolysis reaction mixture into thermostatic heating magnetic stirrer, water bathing at 100 degrees C for 50-70 minutes, and standing at room temperature for 30-40 minutes; taking supernatant, centrifuging at 4000-5000 revolutions/minute for 10-15 minutes, and taking supernatant as hydrolysate; and mixing hydrolysate and vermicompost at 1:30-1:50 mass ratio, adding 50-60 vol.% peat, and stirring using mixer to obtain vegetable cultivation substrate.